Cannula Implantation into the Cisterna Magna in a Mouse Model

Begin with an anesthetized mouse placed in a stereotaxic frame, its head tilted downward to allow easier access to the cisterna magna, a space filled with cerebrospinal fluid or CSF.

A midline scalp incision exposes the cisterna magna, which appears as an inverted triangle between the cerebellum and medulla, covered by the dural membrane.

Use a cotton swab to clean excess fluid from the dura.

Using tweezers, grasp a cannula near the needle portion.

Insert the needle at an angle through the dura into the cisterna magna’s center, avoiding penetration in the cerebellum and medulla.

Clean any leaked CSF.

Apply adhesive to the dura to secure the cannula, then add a glue accelerator for immediate adhesion.

Cover the incision with a mixture of cement and adhesive, followed by a glue accelerator.

The intracisternal cannulated mouse is ready for targeted CSF delivery of therapeutic agents.

Fix the mouse in the stereotaxic frame, making use of either the intraneural arch or the zygomatic arch. Then, tilt the head slightly so that it forms an angle of 120 degrees to the body.

Next, apply ophthalmic ointment and reapply it as needed during the surgery. Find the occipital crest, which is the part of the