Method Article

A Micro-drive Array Method for Electrophysiological Recording from Multiple Brain Regions

July 8th, 2025

In This Article

Abstract

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Source: Shikano, Y., et al. Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat. J. Vis. Exp. (2018).

The video demonstrates simultaneous electrophysiological recording from multiple brain regions in a rat using an integrative micro-drive array. Recording electrodes are placed in the olfactory bulb, reference electrodes in the frontal cortex, and micro-drive array electrodes in the hippocampus. The rat is allowed to forage freely, and simultaneous recording from all the implanted electrodes is recorded using the array.

Protocol

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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of the Integrative Micro-Drive Array

  1. Create a micro-drive array for cortical local field potential (LFP) recordings as described elsewhere. Leave at least 6 metal holes open on an electrode interface board (EIB) for use as electrocardiography/electromyography/breathing rate (ECG/EMG/BR) channels that are connected to bioflex wires as described in 1.2.
  2. Cut a bioflex wire into 6 pieces with lengths of 5.0 cm. Peel off the polytetr....

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Results

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Top view EIB connector diagram; labeled LFP, BR, ECG, EMG for neural and bioelectrical signals.
Figure 1: A top view of the EIB. The EIB includes 24 cortical LFP (LFP) channels that are connected to the tetrodes, 2 ECG channels, 2 EMG channels, 2 BR channels, and 2 ground (Gr) channels. All channels except the LFP channels were connected to insulated w.......

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Disclosures

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No conflicts of interest declared.

Materials

List of materials used in this article
NameCompanyCatalog NumberComments
FEP Hookup Wire Stranded Stainless SteelCooner Wire Company, Chatsworth, CAAS 633Bioflex wire
EIB-36-PTBNeuralynx, Inc., Bozeman, MTEIB-36-PTBEIB
Cereplex MBlackrock Microsystems, Salt Lake City, UTDigital headstage
Cereplex DirectBlackrock Microsystems, Salt Lake City, UTData acquisition system
UEW polyurethane magnet wireOyaide.com, Tokyo, JapanUEW 0.14mm 20mEnamel wire
SD-102Narishige, Tokyo, JapanSD-102High-speed drill
Minimo ONE SERIES ver.2Minitor Co.,Ltd, Tokyo, JapanC2012High-peed drill Power Supply
Provinice 250 mLShofu Inc., Kyoto, Japan213620136Dental cement
Small Animal AnesthetizerBiomachinery, Chiba, JapanTK-7Anesthetizer
Buprenorphine hydrochlorideSigma-Aldrich, St. Louis, MOB7536-1MLAnalgesic
IsofluraneDS Pharma Animal Health, Osaka, JapanIsoflu 250mL
Vaseline, WhiteWako Pure Chemical Industries, Ltd., Osaka, Japan224-00165Vet ointment
Sodium alginateNacalai tesque, Kyoto, Japan31131-85
Calcium Chloride DihydrateWako Pure Chemical Industries, Ltd., Osaka, Japan031-00435
Stainless steel screw M1.0×4.0MonotaRO, Hyogo, Japan42617504Stainless steel screw for BR electrodes
Stainless steel screw M1.4×3.0MonotaRO, Hyogo, Japan42617687Stainless steel screw for g/r electrodes and anchors

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Tags

Micro drive ArrayElectrophysiological RecordingBrain Region RecordingStereotaxic SurgeryElectrode ImplantationDental Cement ApplicationCraniotomy ProcedureAnchor Screw PlacementSignal StabilizationFreely Moving Rat

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