Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

July 1st, 2016

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Summary

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A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure.

The Final Concentration of EGTA in Solution 2 and the Final Concentration of Calcium chloride dihydrate in solutions 3 and 4 have been updated in Table 1.

Abstract

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA 0.1mM

to:

EGTA 1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate 0.5µM

to:

Calcium chloride dihydrate 5mM

Protocol

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA 0.1mM

to:

EGTA 1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate 0.5µM

to:

Calcium chloride dihydrate 5mM

Disclosures

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,
No conflicts of interest declared.

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Tags

Cell IsolationLiver TissueEGTA SolutionCalcium ChlorideTwo step ProcedureHepatocyte IsolationPerfusion TechniqueCell Viability