Osteocytes are highly specialized bone cells with diverse functions, including mechanosensation, regulation of bone resorption, and modulation of mineral metabolism through hormone secretion and signaling. These cells reside within lacunae embedded in a mineralized matrix, with an extensive network of dendritic processes housed in canaliculi that facilitate cell-cell communication and the transport of nutrients and small molecules.

Research suggests that osteocytes actively resorb the surrounding matrix to maintain skeletal integrity and mineral ion homeostasis. Additionally, osteocytes synthesize and secrete key regulatory hormones, such as fibroblast growth factor 23 (FGF23), which controls phosphate homeostasis, and sclerostin, which modulates bone formation. Their role in skeletal diseases is well established, as anti-sclerostin antibodies are used to treat low bone mass disorders, and mutations affecting FGF23 regulation contribute to conditions such as hypophosphatemic rickets.

This collection aims to consolidate a comprehensive set of methods and protocols for the study of osteocyte biology. It will encompass a range of established and emerging techniques, including in vitro osteocyte cell lines, mouse models, and advanced methods for visualizing osteocytes and their lacuno-canalicular network. By bringing together these diverse methodologies, this collection will serve as a valuable resource for researchers investigating osteocyte function and its implications in skeletal health and disease.