Most neurological disorders have both endogenous and exogenous causes. Currently, many of these disorders are incurable due to the lack or partial understanding of the mechanisms behind the various disease and subsequently limited drug development. The establishment of proper disease models is critical for the identification and understanding of cellular and molecular mechanistic aspects. Brain organoids are made up of 3D self-organizing brain tissue, which are generated from human-derived induced pluripotent stem cells (hiPSCs). The use of these models has been proposed to recapitulate various aspects of neurodevelopmental disorders, including the neuronal generation, migration of neurons, and functional cellular networks, which can provide a better understanding into the molecular mechanisms involved in those disorders. To overcome the current limitations of hiPSC-based methods, new and advanced methods are required. This collection procedure will highlight recent methodological advancements in brain organoid technology, including but not limited to genome editing, drug screening, and precise characterization of organoids with the help of cutting-edge technologies. Video-based protocols for recapitulating accurate disease features using functional organoids will be provided with the final objective of generating new therapies for human developmental and neurological disorders.