Guest Editors
Dr. Manash Paul
University of California Los Angeles (UCLA)
Dr. Manash K. Paul is a Principal Investigator and Scientist at the University of California Los Angeles (UCLA). He has contributed significantly to the field of stem cell biology, organoid and disease modeling, regenerative medicine, and lung cancer. His research has focused on various signaling processes involved in maintaining stem cell homeostasis during the injury-repair process, deciphering lung stem cell niche, pulmonary disease modeling, immuno-oncology, organoid bioengineering, and drug discovery. He is currently investigating the role of extracellular vesicles in premalignant lung cell migration, immuno-oncology of the lung, and the use of tumor organoids. Dr. Paul has published in more than 50 peer-reviewed international journals and is highly cited. Dr. Paul is the recipient of many awards, including the UCLA Vice Chancellor’s award, senior member IEEE, and editorial member of many international journals.
Dr. Bharti Bisht
University of California, Los Angeles (UCLA)
Dr. Bharti Bisht, Ph.D. is currently working as a Research Associate at the Department of Thoracic Surgery at David Geffen School of Medicine, University of California, Los Angeles (UCLA), USA. Her research has significantly contributed to lung stem cell biology and regenerative medicine. Dr. Bharti’s research is focused on deciphering signaling pathways regulating murine and human stem cells (SC) lineage specification via deterministic and stochastic gene expression during regeneration after injury. She has gained extensive experience in understanding the mechanism of Adult Stem Cells (ASCs), Embryonic Stem Cells (ESCs), and Induced Pluripotent Stem Cells (iPSCs) in the lung, pancreatic, and bone regeneration, lineage specification, and directed differentiation in both murine and human models. She is an expert in Organoid bioengineering disease modeling and has experience working with lung, pancreas, gut, and bone organoids. Working on a NASA-UCLA collaborative project on Mesenchymal Stem Cells (MSCs) and bone regeneration during space flights had opened an innovative dimension to her quest to understand stem cell and regenerative biology. Her Ph.D. research had provided a strong foundation on insulin signaling and its regulation during diabetes. Dr. Bharti’s research focuses on understanding the cellular and molecular mechanisms of pancreatic stem cell-mediated Beta (β)-cell repair, maintenance, and homeostasis.
Collection Overview
Organoid technology is a multidisciplinary approach that leverages stem cells' capacity for self-renewal, multi-lineage differentiation, and self-organizing into three-dimensional (3D) tissue-mimics called organoids. Organoid culture has fostered the growth of several tissue types, including the kidney, brain, lung, colon, stomach, breast, and liver. The potential for basic and translational research and the associated methodology in developing organ-specific tissue organoids and disease modeling to imitate the physiology and complexity of a tissue-specific illness is excellent. Given the disconnect between animal models and human disease pathology, a paradigm shift toward using a 3D human organoid platform for disease modeling may assist in gaining a better understanding of the human component of a disease.
Organoids provide insight into the process of human disease development, signaling interactions, cell-cell interactions, therapeutic targets, and drug screening and discovery. Genome editing, hybrid culture methods, the establishment of biobanks, and single-cell sequencing are all examples of cutting-edge technology that may aid in the production of more physiologically realistic human disease models, potentially transforming the discovery of novel therapeutics. Because of advancements in biobanking, it has become possible to utilize a person's own stem cells for tailored disease modeling and precision therapy. Organoid for disease modeling is a developing platform with enormous potential for incorporating innovation into future studies.
This collection aims to bring together experts from multiple fields like cancer, fibrosis, neuronal diseases, chronic diseases, infectious diseases, and allied domains to shed light on how organoids are used for disease modeling. A collection of such methodologies can push the boundaries of current scientific research, and future breakthroughs will undoubtedly result in the creation of novel paradigms for combating human diseases.
Articles
Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
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Cited by 16
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2022
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Maxim Le Compte1, Edgar Cardenas De La Hoz2, Sofía Peeters1, Evelien Smits1, Filip Lardon1, Geert Roeyen1,3, Steve Vanlanduit2, Hans Prenen1,4, Marc Peeters1,4, Abraham Lin1,5, Christophe Deben1
1Center for Oncological Research (CORE), Integrated Personalized & Precision Oncology Network (IPPON), University of Antwerp, 2Industrial Vision Lab, University of Antwerp, 3Department of Hepatobiliary Transplantation and Endocrine Surgery, University Hospital Antwerp (UZA), 4Department of Oncology, University Hospital Antwerp (UZA), 5Plasma Lab for Applications in Sustainability and Medicine ANTwerp (PLASMANT), University of Antwerp
Abstracts
Multiplexed live-cell imaging for drug responses in patient-derived organoid models of gynecologic cancer
Kaitriana Powell 1,
Emily Symons1,
Hiruni Sumanasiri1,
Jessica Andrew1,
Emily Witt1,
Kimberly Leslie2,
Lorenzo Buroni3,
Kristina Thiel*1
1University of Iowa,
2University of New Mexico,
3Institute of Cancer Research
Multiplexing brain organoids: longitudinal dissection at single cell resolution of developmental traits
Nicolo Caporale*1
1Human Technopole, University of Milan