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TOPICAL COLLECTIONS

Infection-Relevant Models for Fundamental Microbiology Research and Antimicrobial Drug Development
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Daniel Neill

Daniel Neill

Division of Molecular Microbiology, University of Dundee

Dr. Neill’s lab studies bacterial infections of the human airways. In particular, the lab seeks to understand how the physical, chemical and biological environment of the respiratory tract influences bacterial phenotypes, including those associated with virulence and antimicrobial resistance. The lab focuses on the relationship between upper airway commensalism and lung pathogenesis and aim to define the host and pathogen factors that drive a switch between these two modes of life. Dr. Neill, with his lab strives to improve on existing in vitro and in vivo models of infection, to better capture the key conditions of the respiratory tract in health and disease. A long-term goal is the development of novel therapeutics and vaccines for treatment and prevention of bacterial respiratory diseases.

Miguel Cámara

Miguel Cámara

School of Life Sciences and Biodiscovery Institute, University of Nottingham

Prof. Miguel Cámara is Co-Director of the National Biofilms Innovation Centre (NBIC) working with academic and industrial partners on different areas of biofilm research and innovation. He has many years of expertise in quorum sensing-mediated bacterial cell-cell signalling mechanisms with a particular emphasis on the Cystic Fibrosis opportunistic pathogen Pseudomonas aeruginosa. He has led several drug discovery programs aiming at reducing the impact of antimicrobial resistance through novel anti-virulence target discovery and the design of inhibitors against these, mainly targeting quorum sensing-driven mechanisms. Some research areas he currently studies on include: (i) identification of novel biomarkers of infection for the development of point of care diagnostics; (ii) molecular mechanisms of biofilm development and adaptation to low oxygen conditions; (iv) identification of novel drugs which prevent biofilm formation or sensitise them to antibiotics; (v) mechanisms of interactions between biofilms and the human host.  

Joanne Fothergill

Joanne Fothergill

Department of Clinical Infection, Microbiology and Immunology, University of Liverpool

Dr. Fothergill's research activities are focused on understanding the mechanisms of pathogens, particularly Pseudomonas aeruginosa, in disease. Her work delves into chronic infections. Employing genomics, molecular microbiology, and population studies, she investigates P. aeruginosa's interactions in polymicrobial biofilms, for insights into pathogen evolution. With an expertise in bacterial genomics, molecular epidemiology, and antimicrobial resistance, Dr. Fothergill has developed diagnostic tests for P. aeruginosa. Additionally, she also explores infections caused by Escherichia coli. As deputy director of the Microbiome Innovation Centre at the University of Liverpool, she studies microbiome changes during disease treatment and its impact on pathogen biology.

Collection Overview

Infection is a major cause of global morbidity and mortality. The study of microbial physiology, host-pathogen interactions, and antimicrobial drug development is hampered by the difficulty of capturing the complexity of infection-relevant environments under laboratory conditions. Substantial research has been devoted to developing new model systems and improving upon existing ones, but choosing a model appropriate for a given research question remains a challenge.  

This Methods Collection aims to highlight recent advances in the development and validation of infection-relevant models. We aim to encompass a wide variety of models, ranging from high-throughput, chemically-defined bacterial culture media that reflect host environments to models of host-pathogen interactions and advanced systems for studying drug pharmacokinetics under infection-relevant conditions. 

Articles

Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
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Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis

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Cited by 3

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2024

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Establishing a Microfluidic Co-Culture Blood–Brain Barrier Model Using Human Brain Endothelial Cells and Astrocytes

Establishing a Microfluidic Co-Culture Blood–Brain Barrier Model Using Human Brain Endothelial Cells and Astrocytes

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2026

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Abstracts

Cystic Fibrosis-Specific Hollow Fiber Infection Model (HFIM) for Optimizing Antibiotic Therapy for People with CF

Ashwani Kumar 1,

Nicola Farrington1,

Shampa Das*1,

Joanne L Fothergill2

1Antimicrobial Pharmacodynamics and Therapeutics, University of Liverpool, Liverpool, United Kingdom,

2Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK

Development of a cystic fibrosis aggregate polymicrobial biofilm model to observe changes in virulence gene expression

Tegan Hibbert1,

Hollie Leighton1,

Grace Ritchie1,

Daniel Neill2,

Joanne Fothergill*1

1University of Liverpool,

2University of Dundee

Considerations for use of Galleria mellonella as an antimicrobial screening model

Thomas Barton*1

1University of Dundee

Exploring Tenebrio molitor as a Model for Discovering New Antifungal Agents Against Candidozyma auris

Susana Johann*1,

Aline Dias Valerio1,

Anna Paula Tironi1,

Renata Oliveira1

1Universidade Federal de Minas Gerais