Current Methods for Generating Human Organoids

TOPICAL COLLECTIONS

Guest Editor

You Yu

Soochow University

Dr. Yu is an associate professor at Suzhou Medical College of Soochow University. He completed his Ph.D. at Soochow University in 2019 and continued his academic journey with post-doctoral research until 2022, after which he assumed his current position. Utilizing advanced technologies such as human pluripotent stem cell-derived organoids, gene editing, single-cell sequencing, and ATAC-Seq, his research primarily focuses on the complex functions and regulatory mechanisms of cell fate determination in cardiovascular development and diseases.Dr. Yu has published over 20 papers in peer-reviewed journals, accumulating more than 700 citations and achieving an H-index of 13. He has led several research projects, including serving as the principal investigator for the National Natural Science Foundation for Young Scientists. Additionally, he is an Editorial Board Member for Scientific Reports and has reviewed for journals such as Stem Cell Research & Therapy and Journal of the American Heart Association (JAHA), among others.

Collection Overview

Human organoids provide a more physiologically relevant in vitro model compared to traditional cell lines or animal models. They recapitulate the complex architecture and functions of organs to a certain extent, enabling researchers to investigate the mechanisms of human diseases, drug screening and development, regenerative medicine, as well as basic biological research.

The aim of this section is to expand the knowledge of consolidation and dissemination, create the standardization and comparison, inspire the new ideas and innovation and also provide educational resource for beginners.

Articles

Generation of Human Cardiac Organoids from Embryonic Stem Cells via Stepwise Mesoderm Induction and 3D Self-organization

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2025

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Bingwei Wang^1,2, Chunxiang Zhang^1,2,3,4,5

¹Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education, Southwest Medical University, ²Nucleic Acid Medicine, Key Laboratory of Luzhou, Southwest Medical University, ³Department of Cardiology, The Affiliated Hospital of Southwest Medical University, Southwest Medical University, ⁴Key Laboratory of Medical Electrophysiology, Ministry of Education, Southwest Medical University, ⁵Institute of Cardiovascular Research, Southwest Medical University

4:37

Generation of Human Induced Pluripotent Stem Cell-derived Planar Hair-bearing Skin Organoids Using an Air-Liquid Interface Culture System

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Cited by 1

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2025

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Charlotte M. S. De Henau^1,2, Victor Lorrain^1,2, Myrthe P. Flesseman^1,2, Veronika Ramovs^1,2, Karine Raymond^1,2,3

¹Department of Anatomy and Embryology, Leiden University Medical Center, ²The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, ³CEA, Inserm, IRIG, UA13 BGE, Biomics, University of Grenoble Alpes

10:14

Generation of 3D Collagen-Embedded A549/MRC-5 Alveolar Organoids using a Mechanical Cell-Stretching Bioreactor

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2026

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Tony J. F. Guo*^1,2, Safiya Al Yazeedi*¹, Janaeya Z. Baher¹, Logan Yee¹, Chung Cheung², Don D. Sin², Emmanuel T. Osei^1,2

¹Department of Biology, University of British Columbia - Okanagan, ²Centre for Heart Lung Innovation, St. Paul's Hospital, University of British Columbia

Abstracts

Creating 3D organoids from isolated human pancreatic ductal cells

Habib Rezanejad^*1

¹Assistant Professor at MacEwan University

3D and 2D Intestinal organoids to study effects of genetic mutations on epithelial physiology and function

Francesca Di Cara^*1

¹Dalhousie University

Generation of hiPSC-Derived Hair-Bearing Skin Organoids in Cystic and Planar Configurations.

Karine Raymond^*1

¹Leiden University Medical Center

Organoid Radiation Survival Assay for Prostate Cancer Patient-derived Cell line CaB34 and paired Radioresistant Subline CaB34-CF

Jessica Wright^*1

¹Sunnybrook Research Institute

A novel organoid-derived transwell model to investigate intestinal epithelial- immune cell interactions

Laura Blum¹,

Makenzie Jones¹,

Michael Boettcher¹,

Jasmin Knopf^*1

¹1 Department of Pediatric Surgery, University Medical Center Mannheim, University Heidelberg, Theodor- Kutzer-Ufer 1-3, 68167 Mannheim. 2 Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany