
Organoids are a widely used tool for modeling human epithelial organs and are revolutionizing modern medicine with their wide ranging applications. These applications include disease modeling, drug development and discovery, personalized medicine, gene editing, modeling human development, and developing novel cell therapies. Organoids are miniature 3D tissues that resemble the cellular hierarchy and retain cellular heterogeneity that is present in the original tissue of interest. Organoids are derived from human pluripotent stem cell-derived progenitors or tumor-derived cells and can not only mimic tissue structure but also tissue function. Within these organoids, biological mechanisms like stem cell self-renewal, differentiation, and self-organization drive organoid formation; but also less appreciated biomechanical, biophysical, and engineering principles have influence over outcomes. Methods to engineer organoids are non-obvious and are highly dependent upon classic wet-laboratory techniques including the use of non-adherent microwell plates; complex extracellular matrices; and careful handling, visualization, and manipulation. In this collection, we invite video-based articles that highlight details of methods for engineering organoids or their applications.
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Cited by 2
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2021
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1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)
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Cited by 2
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2022
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2025
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Simran Kumar1, Jenna Venturo1, Helly Patel1, Lokprakash Aravindan3, Kyle Leatt3, Natesh Parashurama1,2,3,4,5
1Department of Biomedical Engineering, University at Buffalo (State University of New York), 2Clinical and Translation Research Center (CTRC), University at Buffalo (State University of New York), 3Department of Chemical and Biological Engineering, University at Buffalo (State University of New York), 4Center for Cell, Gene and Tissue Engineering, University at Buffalo, State University of New York, 5Khufu Therapeutics, Inc.
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Cited by 2
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2021
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1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina
Methods for Hepatic-Mesenchymal Assembloid Formation
Simran Kumar1,
Helly Patel1,
Kirsten Mcgraw1,
Natesh Parashurama*1
1University at Buffalo
COMPARATIVE STUDY OF COMMERCIALLY AVAILABLE BASEMENT-MEMBRANE MATRICES FOR HUMAN STEM CELL MAINTENANCE AND INTESTINAL ORGANOID GENERATION
Janny Pineiro Llanes*1,
Lais da Silva1,
Rodrigo Cristofoletti*1
1Center for Pharmacometrics & Systems Pharmacology, Department of Pharmaceutics, University of Florida