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High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

DOI:
10.3791/62668-v

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06:25 min

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June 14, 2021

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, , , , , , ,
1FUJIFILM Wako Bio Solutions Corporation, 2Medical-Industrial Translational Research Center,Fukushima Medical University, 3Department of Bioregulation and Pharmacological Medicine,Fukushima Medical University

Chapters

  • 00:04Introduction
  • 00:59Patient-Derived Tumor Organoid Culture
  • 02:25High Throughput Assay System for Growth Inhibition
  • 03:32High Throughput Assay System with Cell Picking and Imaging for Growth Inhibition
  • 04:30Results: Estimation of Anticancer Effects on the Patient-Derived Tumor Organoids
  • 05:55Conclusion

Summary

Automatic Translation

Automatic Translation

A highly accurate in vitro high-throughput assay system was developed to evaluate anticancer drugs using patient-derived tumor organoids (PDOs), similar to cancer tissues but are unsuitable for in vitro high-throughput assay systems with 96-well and 384-well plates.

Tags

Keywords: Patient-derived Tumor OrganoidsHigh-throughput In Vitro AssayCell Analysis2D Culture3D CultureMolecular Targeted DrugsCell Suspension384-well MicroplateLiquid HandlerCell Fragmentation And Dispersion Instrument

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