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TOPICAL COLLECTIONS

Primary Cell-, Stem cell- and Tumor-derived Spheroid and Organoid Cultivation Techniques

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Natesh Parashurama

Natesh Parashurama

University at Buffalo - State University of New York, Department of Chemical and Biological Engineering; University at Buffalo - State University of New York, Department of Biomedical Engineering and School of Medicine

Natesh Parashurama, MD PhD is a physician scientist, bioengineer, and full time researcher. He received his BS in chemical and bio engineering from MIT, his MD from University at Buffalo, and his PhD from Rutgers University/ Harvard Medical School. He studied hepatic tissue engineering under Professor Mehmet Toner (Harvard) as an undergraduate at MIT, and after training in General Surgery, for his doctoral thesis, he investigated liver stem cell and tissue engineering, synthetic and gene regulatory biology under Professor Martin L. Yarmush at Rutgers University (Coursework) and Harvard Medical School and Massachusetts General Hospital (MGH) (Research). He then studied multimodality molecular imaging, reporter gene imaging, and diagnostics under Professor Sanjiv Gambhir (Stanford). He was an instructor and California Institute of Regenerative Medicine (CIRM) Human Development under Susan Fisher (UCSF) before joining the University at Buffalo, State University of NY in 2016. He has given several invited talks, and he has mentored over 24 undergraduates, 14 graduate students, his researched has been featured on the cover of Stem cells, and he has won a Young investigator award from the International Society of Cell Therapy (ISCT), a best manuscript award from the Radiological Society of North America (RSNA), and a department mentoring award for mentoring minority students.

Collection Overview

Organoids are a widely used tool for modeling human epithelial organs and are revolutionizing modern medicine with their wide ranging applications. These applications include disease modeling, drug development and discovery, personalized medicine, gene editing, modeling human development, and developing novel cell therapies. Organoids are miniature 3D tissues that resemble the cellular hierarchy and retain cellular heterogeneity that is present in the original tissue of interest. Organoids are derived from human pluripotent stem cell-derived progenitors or tumor-derived cells and can not only mimic tissue structure but also tissue function. Within these organoids, biological mechanisms like stem cell self-renewal, differentiation, and self-organization drive organoid formation; but also less appreciated biomechanical, biophysical, and engineering principles have influence over outcomes. Methods to engineer organoids are non-obvious and are highly dependent upon classic wet-laboratory techniques including the use of non-adherent microwell plates; complex extracellular matrices; and careful handling, visualization, and manipulation. In this collection, we invite video-based articles that highlight details of methods for engineering organoids or their applications.

Articles

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
10:51

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

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Cited by 6

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2021

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Abstracts

Methods for Hepatic-Mesenchymal Assembloid Formation

Simran Kumar1,

Helly Patel1,

Kirsten Mcgraw1,

Natesh Parashurama*1

1University at Buffalo

COMPARATIVE STUDY OF COMMERCIALLY AVAILABLE BASEMENT-MEMBRANE MATRICES FOR HUMAN STEM CELL MAINTENANCE AND INTESTINAL ORGANOID GENERATION

Janny Pineiro Llanes*1,

Lais da Silva1,

Rodrigo Cristofoletti*1

1Center for Pharmacometrics & Systems Pharmacology, Department of Pharmaceutics, University of Florida