
Beata Greb-Markiewicz
Wroclaw University of Science and Technology, Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry
Dr. Beata Greb-Markiewicz is an Associate Professor in the Department of Biochemistry, Molecular Biology, and Biotechnology in the Faculty of Chemistry at Wroclaw University of Science and Technology (Wroclaw, Poland). Her research interest is focused on the family of bHLH transcription factors, with a special interest in bHLH-PAS proteins. She is interested in proteins intrinsic disorder, protein-protein interactions, liquid-liquid phase separation, protein localization, and signal transduction.Dr. Greb-Markiewicz prepared her PhD thesis titled “Searching for factors engaged in infection and intoxication process of the insect by entomopathogenic fungi from Zoophthora and Paecilomyces genera” in the Laboratory of Ecological Chemistry in the Wroclaw University of Technology. She did her post-graduate research in “Management of research project and commercialization of research results” at the Wroclaw University of Technology.Dr. Greb-Markiewicz carried out her two-year post-doctoral research at the Ulm University at The Institute of General Zoology and Endocrinology (Ulm, Germany) and a short research training at the Institutionen för Växtvetenskap Swedish University of Agricultural Sciences (Alnarp, Sweden) and the Istituto per la Chimica di Molecole di Interesse Biologico (Napoli, Italy). She was also a guest scientist at the Max-Planck Institute of Molecular Cell Biology and Genetics with the Susanne Eaton group (Dresden, Germany). She is a publishing author and reviewer for various internationally recognized journals. She has participated in well-known international conferences organized by EMBL and FEBS, and courses organized by EMBL and Pasteur Institute.In addition to her research, Beata is passionate about art. She is a classically trained violinist performing concerts with maritime folk music groups.

Aneta Tarczewska
Wroclaw University of Science and Technology, Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry
Dr. Aneta Tarczewska is an Assistant Professor in the Department of Biochemistry, Molecular Biology and Biotechnology, Wroclaw University of Science and Technology since 2016. She studied Biomedical Science at Robert Gordon University in Scotland. She completed her graduate project at the University of Palermo. Next, she finished postgraduate studies in Instrumental Analytical Science at The Robert Gordon University. She obtained her PhD in biochemistry at Wroclaw University of Science and Technology. Her thesis titled “Analysis of structural complexity of Chd64 and FKBP39 from Tribolium castaneum” presents the results of the analysis of proteins involved in the regulation of a dynamic multiprotein complex that regulates gene expression in insect cells. Dr. Tarczewska is focused on understanding the structure-function relationship of intrinsically disordered proteins associated with gene regulation processes. She is particularly interested in the properties of proteins belonging to FK-506 immunophilins and bHLH family of transcription factors. Dr. Aneta Tarczewska is a publishing scientist, participating in various international conferences. She also serves as a reviewer in internationally recognized journals. She improves her qualifications continuously by participating in a wide range of training and courses. She is a member of the Polish Biochemical Society, contributing to the popularization of science.

Marta Kolonko-Adamska
Wroclaw University of Science and Technology, Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry
Dr. Marta Kolonko-Adamska received her PhD in the field of Chemistry from the Wroclaw University of Science and Technology (Poland) in 2020. Her PhD thesis was titled “Molecular analysis of the C-terminal fragments of Methoprene tolerant and Germ cell-expressed receptors from Drosophila melanogaster”. During her studies, Dr. Kolonko-Adamska did multiple research internships with Professor M. Sattler in Helmholtz Zentrum München (Germany) at the Institute of Structural Biology. She was part of projects aimed at rational drug discovery. The results were published in a high impact factor journal (Science, J Med Chem.). Since 2018 she is the leader of two grants financed by National Science Center Poland (NCN). Currently, she is an Assistant Professor at the Department of Biochemistry, Molecular Biology, and Biotechnology, Faculty of Chemistry, Wroclaw University of Science and Technology (Poland). Her research interests focus mainly on the structural biochemistry, structure-function relationship of intrinsically disordered proteins, transcription regulation, proteins interactions, and liquid-liquid phase separation analysis.
For a long time, the main existing paradigm of biochemistry and structural biology stated that the functionality of protein results directly from its defined tertiary structure. Currently, almost one-third of eukaryotic proteins, characterized by the lack of a stable tertiary structure and engaged in crucial biological processes, were shown to be fully functional. In literature, such proteins are known as intrinsically disordered proteins (IDPs). Importantly, most of the proteins participating in signal transduction comprise long intrinsically disordered regions (IDRs). The structural flexibility and the ability to adopt different conformational states are beneficial in molecular recognition processes, which allow IDPs/IDRs to interact with several partners and to act as hubs in different signaling cascades. Due to the lack of a single stable structure, their detection and characterization can be challenging.
This Methods Collection aims to summarize current methods used in intrinsically disordered proteins/regions studies. This covers a selection of articles presenting both basic and advanced techniques that can be used for the determination of structural and functional properties of disordered proteins. Moreover, techniques dedicated to studies of disordered proteins' interaction with physiological partners (proteins, ligands, nucleic acids), the role of their post-translational modification, and the ability to drive liquid-liquid phase separation are included.
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Cited by 6
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2024
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Estimation of the structural sensitivity of intrinsically disordered regions in response to hyperosmotic stress in living cells using FRET
Constanza Enriquez Toledo 1,
Cesar Ponce Diego 1,
Cesar Luis Cuevas Velazquez*1
1Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, México
Disulfide-bond treatment in computational studies of proteins
Pamela Smardz1,
Pawel Krupa*1
1Institute of Physics, Polish Academy of Sciences