Microrna Lentivirus
Microrna Lentivirus - Related Videos
09:06
Stereotactic Injection of MicroRNA-expressing Lentiviruses to the Mouse Hippocampus CA1 Region and Assessment of the Behavioral Outcome
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Cited by 23 •
2013
MicroRNAs have significant roles in brain structure and function. Here we describe a method to enforce hippocampal miRNA over-expression using stereotactic injection of an engineered miRNA-expressing lentivirus. This approach can serve as a relatively rapid way to assess the in vivo effects of over-expressed miRNAs in specific brain regions.
11:42
Lentivirus Production
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Cited by 43 •
2009
To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.
01:22
MicroRNAs
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2019
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
07:23
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Cited by 3 •
2016
Herein we propose a strategy to study the effect of a transcription factor of interest on the microRNA transcriptome using publically available data, computational resources and high throughput data from microRNA arrays after transfecting cells with small hairpin (sh)RNA targeting a transcription factor of interest.
03:31
Generation of Recombinant Lentivirus in Mammalian Cells
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2026
Source: Zhang, Y., et al., Direct Reprogramming of Mouse Fibroblasts into Melanocytes. J. Vis. Exp. (2021)This video demonstrates the production and concentration of recombinant lentivirus in mammalian cells, enabling efficient gene delivery of transcription factors or other genetic elements for applications such as cellular reprogramming, functional studies, and potential therapeutic research.
FAQs
Once the lentiviral vector delivers the sequence, the cell transcribes it as an RNA precursor and processes that transcript into mature microRNA. The mature molecule then associates with the RNA-induced silencing complex, or RISC, which provides the functional machinery for recognizing complementary messenger RNA. This cellular processing connects vector delivery to downstream gene regulation.
The mature microRNA guides RISC toward messenger RNA containing a complementary sequence. After binding, the targeted transcript may be translated less efficiently or may undergo degradation, reducing expression of the corresponding gene. Thus, the sequence carried by the vector is central to which messenger RNA is regulated and how strongly gene output is altered.
Sustained expression allows gene regulation to persist beyond an initial delivery period, making the approach suitable for long-term studies. In behavioral research, this persistence can support investigation of how altered gene activity relates to processes such as learning, memory, motivation, and stress responses rather than limiting analysis to short-lived cellular effects.
A typical workflow begins by introducing the lentiviral vector into cultured cells or an animal model. The delivered sequence is then transcribed and processed by the host cell, after which the resulting microRNA regulates complementary messenger RNA. Researchers can use this sustained change in gene activity to examine molecular effects and behavior in the selected model.
By altering gene activity in defined neural circuits, this approach provides a way to examine whether molecular changes are associated with behavioral outcomes. Researchers can relate circuit-level regulation to functions including learning, memory, motivation, or stress responses. The method therefore links a targeted gene-regulatory manipulation with broader behavioral observations in an animal model.
Cultured cells provide a setting for studying how delivered microRNA sequences are transcribed, processed, and used to regulate messenger RNA. Animal models extend the investigation to neural circuits and behavior. Because expression can persist, the same general strategy supports long-term analysis of gene function across cellular systems and behaviorally relevant models.