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Chapters
- 00:05Title
- 02:13Protein Extraction
- 03:03Endoproteinase LysC and Tryptic Protein Digestion of Whole Lysate Using Filter Assisted Sample Preparation
- 05:34Ion Exchange Chromatography
- 06:20GELFREE Fractionation of Cochlear Sensory Epithelium
- 07:54Protein Digestion of GELFREE Fractions Using Filter Assisted Sample Preparation
- 10:07Results: Identification of membrane and Soluble Proteins by High-resolution Mass Spectrometry Analysis
- 14:00Conclusion
Proteome analysis of the cochlear sensory epithelium can be challenging due to its small size and because membrane proteins are difficult to isolate and identify. Both membrane and soluble proteins can be identified by combining multiple preparative methods and separation techniques along with high-resolution mass spectrometry.
