Calcium Green-5n
Calcium Green-5n - Related Videos
03:13
Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria
0 Views •
2025
This video describes a plate reader-based assay to measure mitochondrial calcium uptake in isolated mitochondria. The assay analyzes the fluorescence signal of a calcium-sensitive fluorescent dye to study the kinetics of mitochondrial calcium uptake and calcium overload.
04:46
Calcium Influx Assay to Measure Mitochondrial Calcium Uptake in Cultured Cells
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2025
This video describes a confocal microscopy imaging-based assay to measure mitochondrial calcium uptake in cultured cells. The assay uses the calcium-sensitive fluorescent dye Rhod-2/AM to measure the mitochondrial calcium in permeabilized cultured cells.
12:10
Measuring Fast Calcium Fluxes in Cardiomyocytes
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Cited by 3 •
2011
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
08:43
Calcium Imaging in Neurons
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2023
Calcium ions play an integral role in neuron function: They act as intracellular signals that can elicit responses such as altered gene expression and neurotransmitter release from synaptic vesicles. Within the cell, calcium concentration is highly dynamic due to the presence of pumps that selectively transport these ions in response to a variety of signals. Calcium imaging takes advantage of intracellular calcium flux to directly visualize calcium signaling in living neurons.This video begins...
01:21
Green Algae
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2025
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
FAQs
The signal changes when Ca2+ binds to calcium-binding sites within the dye. This binding alters the dye’s fluorescent behavior, producing an increase that can be measured optically. Because the response is linked to binding rather than merely to the presence of calcium in a sample, fluorescence changes provide a way to follow shifts in ion availability during biochemical or cellular experiments.
A relatively low-affinity indicator is less suited to very low calcium levels but remains responsive when free Ca2+ concentrations are elevated. Calcium Green-5N can therefore report changes across higher calcium ranges without becoming fully unresponsive as readily as a higher-affinity indicator might. This characteristic is important when studying calcium-rich solutions, organelles, or experimental preparations.
Its fluorescence responds to Ca2+ binding, so measured intensity changes are related to the fraction of calcium available to interact with the indicator. This makes the dye useful for examining free calcium rather than treating all calcium in a preparation as equivalent. In biochemistry, that distinction supports analysis of ion transport, buffering, and calcium-dependent reactions.
The indicator can be applied to biochemical and cellular systems in which calcium levels are sufficiently elevated for its response range. Supported settings include defined solutions, isolated organelles, and other experimental preparations. Selecting among these systems allows researchers to examine calcium behavior under controlled conditions or within structures where transport and buffering influence the measured optical signal.
Time-dependent fluorescence changes can reveal whether free Ca2+ availability rises or falls during an experiment, providing a readout of calcium flux. Researchers can use these measurements to characterize ion movement and buffering behavior, then relate the changes to calcium-dependent processes. The approach is especially relevant when the preparation undergoes substantial changes within an elevated calcium range.
Calcium homeostasis depends on controlling the availability of free Ca2+ across cellular and subcellular environments. By reporting fluorescence changes after calcium binding, Calcium Green-5N helps investigators examine how transport and buffering shape that availability. These measurements provide biochemical context for studies of cellular signaling, muscle function, isolated organelles, and other calcium-dependent experimental systems.