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Measuring Fast Calcium Fluxes in Cardiomyocytes

Measuring Fast Calcium Fluxes in Cardiomyocytes

Article DOI: 10.3791/3505
November 29th, 2011


Summary November 29th, 2011

We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.

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