Microplate Luminescence Counter
Microplate Luminescence Counter - Related Videos
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Introduction to the Microplate Reader
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The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments on the plate reader utilize a standard curve to determine the experimental values. This curve uses samples of known...
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Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons
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In this video, we describe a luminescence-based method to assess the neurotoxicity of neurite outgrowth-promoting small molecule compounds on the viability of hNPC-derived neurons. The assay measures intracellular ATP level, an indicator of cell viability, to evaluate neurotoxicity following test compound treatment.
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Determining Cell Number During Cell Culture using the Scepter Cell Counter
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The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
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Expression of the Luminescence Operon in Marine Bioluminescent Bacteria
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Source: Brodl, E., et al. In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria. J. Vis. Exp. 1 (2018)This video demonstrates the cultivation of marine bioluminescent bacteria to observe quorum-sensing–mediated activation of the luminescence operon, enabling real-time monitoring of gene expression and light emission for studies in microbial communication and gene regulation.
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Precise and High-Throughput Analysis of Bacterial Growth Using a Microplate Reader
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Source: Kurokawa, M., et al. Precise, High-throughput Analysis of Bacterial Growth. J. Vis. Exp. (2017)This video demonstrates the high-throughput quantification of bacterial growth using a microplate reader. It outlines the steps involved in culture preparation, optical density measurement, and analysis of growth dynamics.
FAQs
The instrument collects photons emitted from each well with a sensitive photodetector, such as a photomultiplier tube. It converts the detected light intensity into a quantitative signal that can be compared across samples. This measurement approach allows researchers to relate luminescence produced by a biochemical or biological reaction to the process being investigated.
Programmed reagent addition helps introduce reaction components in a controlled sequence, while timed integration defines when emitted light is collected. Together, these functions coordinate reaction initiation and detection across wells. Consistent timing supports comparable measurements among samples and helps the counter capture signals from reactions whose light output is measured after reagent exposure.
Measurements can be performed across many wells using small sample volumes, allowing numerous reactions or conditions to be assessed in a single plate. Automation further reduces repeated manual handling and improves experimental efficiency. These features are valuable when researchers need to compare multiple samples while detecting light signals from molecular or cellular processes.
Researchers place prepared samples in a multiwell plate, arrange the relevant reaction conditions, and program reagent addition when the assay requires it. The counter then measures light from individual wells, often using a defined integration period, and records the resulting signals for comparison. This workflow supports parallel analysis rather than measuring samples one at a time.
A Microplate Luminescence Counter can support measurements of ATP, enzyme activity, reporter gene expression, cell viability, and immunoassay signals. These applications use luminescence as an indicator of a specific biochemical or cellular event. The resulting quantitative signals help researchers evaluate reaction activity, cellular status, or assay responses across multiple samples.
Researchers may choose this approach when they need sensitive detection across many samples and want to conserve sample volume. In biochemistry, it can examine molecular activities such as enzyme reactions or reporter expression, as well as cellular outcomes such as viability. Its compatibility with automation also supports efficient analysis of large experimental sets.