Arterial Graft Fabrication
Arterial Graft Fabrication - Related Videos
06:55
Fabrication of Small Caliber Stent-grafts Using Electrospinning and Balloon Expandable Bare Metal Stents
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Cited by 10 •
2016
In the protocol, we present a method to manufacture a small caliber stent-graft by sandwiching a balloon expandable stent between two electrospun nanofibrous polyurethane layers.
15:21
Training a Sophisticated Microsurgical Technique: Interposition of External Jugular Vein Graft in the Common Carotid Artery in Rats
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Cited by 9 •
2012
Neointimal hyperplasia is the primary cause of stenosis in arterialized veins. We propose a new protocol whereby the right external jugular vein is grafted using the cuff technique in the common carotid artery of Sprague Dawley rats. The survival rate was 100 % at the time point of sacrifice.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
16:00
Fabrication of the Thermoplastic Microfluidic Channels
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2008
Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.
FAQs
Biomaterials and scaffold architecture influence the balance between mechanical strength, flexibility, and blood-contacting surface characteristics. These design choices affect how the construct maintains structural integrity and interacts with blood and surrounding tissue. Consequently, Arterial Graft Fabrication requires coordinated materials selection and scaffold design rather than treating either factor in isolation.
Some approaches incorporate vascular cells, while others add controlled bioreactor conditioning. These features extend fabrication beyond producing a scaffold: cells provide a tissue-engineering component, and conditioning introduces a controlled preparation step. Their use is approach-dependent, allowing researchers to compare designs with different combinations of biomaterials, cells, and conditioning.
Evaluation should include structural integrity, permeability, thrombogenicity, and compatibility with surrounding tissue. Each measure addresses a distinct performance dimension: integrity concerns structural soundness, permeability concerns transport through the graft, thrombogenicity concerns clot-promoting behavior, and compatibility concerns interaction with adjacent tissue. Together, these measures provide a multidimensional assessment rather than a single mechanical or biological result.
A fabrication workflow can begin with selecting biomaterials and designing a scaffold, followed by creating the vascular substitute. Depending on the approach, vascular cells may be incorporated, and the construct may undergo controlled bioreactor conditioning. Researchers then assess structural integrity, permeability, thrombogenicity, and compatibility with surrounding tissue. This sequence connects construction, optional tissue-engineering steps, and performance evaluation.
Development of fabricated grafts is especially relevant when bioengineers seek alternatives to autologous vessels for cardiovascular surgery. The work does not replace the need to assess graft properties; instead, it creates candidate substitutes that can be examined for structural integrity, permeability, thrombogenicity, and tissue compatibility. This connects material innovation with a clinically important application.
These constructs function as research platforms rather than only potential surgical substitutes. They enable investigation of vascular remodeling, graft failure, and regenerative medicine. A fabricated system can therefore connect scaffold and tissue-engineering decisions with questions about how grafts change, why they fail, and how regenerative approaches may be developed.