Exosome Cargo Analysis
Exosome Cargo Analysis - Related Videos
11:09
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
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Cited by 8 •
2014
Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.
11:02
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement (EFIRM)
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Cited by 43 •
2015
Exosomes are microvesicular structures found within biofluids that potentially carry important disease discriminatory biomarkers. Here, a novel method is used to specifically extract exosomes and rapidly test the exosomal cargo for both RNA/protein targets following the disruption of exosomes using non-uniform electric cyclic square waves.
02:38
Lipopolysaccharide-Induced Acute Lung Injury in Mice for Exosome and Immune Analysis
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2026
Source: Yuan, Z., et al. Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury. J. Vis. Exp. (2018)This video demonstrates an in vivo mouse model for inducing and analyzing acute lung injury (ALI) using intraperitoneal administration of bacterial lipopolysaccharide. The inflammatory cascade triggered by alveolar macrophages and neutrophils leads to epithelial and endothelial barrier disruption, allowing fluid and immune cells to accumulate in the alveoli. Alveolar...
08:06
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
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Cited by 1 •
2016
Here, a protocol is presented to identify Kinesin-1 cargos. A motorless mutant of the Kinesin-1 heavy chain (KIF5B) aggregates in the cytoplasm and induces aggregation of its cargos. Both aggregates are detected under fluorescence microscopy. A similar strategy can be employed to identify cargos of other motor proteins.
04:21
Exosome Isolation: A Technique to Separate Exosomes from the Plasma of Non-small Cell Lung Cancer Patients
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2023
The plasma-isolated exosomes can be used as a non-invasive and repeatable way for the detection and follow-up of the biomarkers for early detection and disease progression. This video describes the protocol for the separation of exosomes from the plasma samples of non-small cell lung cancer patients, which could be used for DNA and protein extractions.
FAQs
The cargo profile provides a molecular basis for vesicle-mediated communication. Examining proteins, lipids, nucleic acids, and other contents together can reveal how one cell may influence tissue behavior, disease processes, or immune responses. In bioengineering, these profiles support comparison of native vesicles and help evaluate whether engineered exosomes carry components relevant to a desired biological effect.
Identity and purity checks establish that the measured molecules come from the intended exosome preparation. This foundation is important when comparing samples from biological fluids or cell cultures, because an inadequately characterized preparation can weaken conclusions about signaling or biomarkers. The checks also support reliable evaluation of engineered vesicles and their intended cargo.
These approaches provide complementary ways to characterize molecular contents. Sequencing, mass spectrometry, and targeted molecular assays can be selected according to the cargo or question being investigated, while together they broaden characterization across nucleic acids, proteins, lipids, and other components. Their results help build cargo profiles for biological interpretation or engineering decisions.
Cargo analysis examines whether engineered exosomes contain the intended molecular material, retain it over time, and produce relevant functional effects. Assessing loading addresses the amount or presence of cargo introduced, while stability indicates whether that cargo remains associated with the vesicles. Functional evaluation connects the molecular design to its effects in bioengineering applications.
A workflow begins by isolating exosomes from a biological fluid or cell culture. Researchers then verify vesicle identity and purity before characterizing the cargo with sequencing, mass spectrometry, or targeted molecular assays. Organizing the process in this order helps ensure that subsequent cargo measurements are interpreted from a defined preparation rather than from unverified material.
The approach is useful when researchers need to connect vesicle contents with tissue behavior, disease processes, or immune responses, or when they are developing engineered delivery systems. It supports biomarker development, therapeutic delivery, and regenerative medicine. Cargo measurements also provide a way to assess whether engineered exosomes achieve the intended loading, stability, and functional outcome.