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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis

9,341 Views

11:09 min

October 30, 2014

DOI:

11:09 min
October 30, 2014

9329 Views
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Summary

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Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.

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