Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Sylvia Neumann; George E. Campbell; Lukasz Szpankowski; Lawrence S.B. Goldstein; Sandra E. Encalada

doi:10.3791/52029

Journal

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Neuroscience

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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using “Cargo Mapping” Analysis

9,341 Views

•

11:09 min

•

October 30, 2014

DOI:

10.3791/52029-v

DOI:

10.3791/52029-v

•

11:09 min

October 30, 2014

•

9329 Views

Sylvia Neumann¹, George E. Campbell*¹, Lukasz Szpankowski*^2,3, Lawrence S.B. Goldstein^2,4, Sandra E. Encalada¹

¹Department of Molecular and Experimental Medicine, Dorris Neuroscience Center,The Scripps Research Institute ²Department of Cellular and Molecular Medicine,University of California San Diego ³Department of Bioengineering,University of California San Diego ⁴Department of Neurosciences,University of California San Diego School of Medicine

Summary

Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.