Hierarchical Vascular Network
Hierarchical Vascular Network - Related Videos
03:33
Visualization of Neural and Vascular Networks in a Chicken Embryo
0 Views •
2025
Source: Delalande, J., et.al. Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using ChickGFP Neural Tube Grafting and Carbocyanine Dye DiI Injection. J. Vis. Exp. (2015)This video demonstrates the transplantation of a GFP-labeled donor neural tube from a stage-matched transgenic chicken embryo into a recipient embryo at the level of somites one to seven, followed by vascular labeling using a lipophilic fluorescent dye. The combined approach allows for direct...
10:53
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
0 Views •
Cited by 10 •
2017
This protocol outlines the implementation of image-guided, laser-based hydrogel degradation to fabricate vascular-derived, biomimetic microfluidic networks embedded in poly(ethylene glycol) diacrylate (PEGDA) hydrogels. These biomimetic microfluidic systems may be useful for tissue engineering applications, generation of in vitro disease models, and fabrication of advanced "on-a-chip" devices.
07:05
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
0 Views •
Cited by 10 •
2018
The protocol describes how to engineer a perfusable vascular network in a spheroid. The spheroid's surrounding microenvironment is devised to induce angiogenesis and connect the spheroid to the microchannels in a microfluidic device. The method allows the perfusion of the spheroid, which is a long-awaited technique in three-dimensional cultures.
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
0 Views •
Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
07:56
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo
0 Views •
Cited by 13 •
2014
Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.
FAQs
Repeated branching distributes fluid from larger conduits toward smaller vessels, while changes in diameter help organize movement across the network’s multiple scales. Interconnected pathways provide more than a single route, supporting regulated flow and shortening the distances over which transported substances must reach tissue. Together, these architectural features help maintain delivery and exchange throughout the construct.
A single pathway can limit distribution if transport must follow one route. Interconnections create alternate routes through the architecture, while repeated branching transfers flow across vessel scales. This organization helps reduce transport distances within engineered tissue and supports more consistent access to regions that depend on circulating fluid for delivery and waste removal.
Large arteries and veins provide the outer scale of distribution, whereas microscopic capillaries bring the network closer to individual cells. This scale progression links circulation with local exchange, allowing nutrients and oxygen to move toward tissue while waste moves away. In engineered systems, reproducing that progression is important because delivery and removal must occur throughout the construct, not only at its surface.
Bioengineers apply repeated branching, diameter changes, and interconnected pathways when designing engineered tissues, organ-on-a-chip platforms, or biomimetic materials. The goal is not merely to create channels, but to recreate the architectural relationships that regulate flow and reduce transport distances. These design choices can improve nutrient delivery, oxygen transport, and waste removal in the engineered setting.
Applications span engineered tissues, organ-on-a-chip platforms, and biomimetic materials. Across these settings, the network architecture provides a framework for studying or supporting fluid distribution and tissue exchange. Although the formats differ, each can use multiscale branching and interconnected pathways to address transport requirements and improve the physiological relevance of the resulting model or construct.
A functional hierarchy can help larger tissue constructs receive nutrients and oxygen while removing waste, addressing transport demands that become important as constructs grow. This makes vascular design central to developing clinically relevant engineered tissues. In regenerative medicine, these principles support efforts to build tissue models and biomimetic materials that better reproduce the transport role of native circulation.