Photoinitiated Gelation
Photoinitiated Gelation - Related Videos
07:26
Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
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Cited by 6 •
2023
Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.
07:28
3D Printing and In Situ Surface Modification via Type I Photoinitiated Reversible Addition-Fragmentation Chain Transfer Polymerization
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2022
The present protocol describes the digital light processing-based 3D printing of polymeric materials using type I photoinitiated reversible addition-fragmentation chain transfer polymerization and the subsequent in situ material post-functionalization via surface-mediated polymerization. Photoinduced 3D printing provides materials with independently tailored and spatially controlled bulk and interfacial properties.
06:31
Highly Stereoselective Synthesis of 1,6-Ketoesters Mediated by Ionic Liquids: A Three-component Reaction Enabling Rapid Access to a New Class of Low Molecular Weight Gelators
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Cited by 4 •
2015
Ionic liquids (ILs) mediate fast, simple and cheap access to 1,6-ketoesters in high diastereoselectivities and good yields. The reaction protocol is robust and the 1,6-ketoesters can be obtained in gram scale after a simple filtration protocol. Moreover, the 1,6-ketoesters are potent gelators in hydrocarbon solvents.
08:22
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
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Cited by 37 •
2017
We provide a generalized protocol based on a microfluidic bioprinting strategy for engineering a microfibrous vascular bed, where a secondary cell type could be further seeded into the interstitial space of this microfibrous structure to generate vascularized tissues and organoids.
10:45
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
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Cited by 26 •
2016
We describe a sequential process for light-mediated formation and subsequent biochemical patterning of synthetic hydrogel matrices for three-dimensional cell culture applications. The construction and modification of hydrogels with cytocompatible photoclick chemistry is demonstrated. Additionally, facile techniques to quantify and observe patterns and determine cell viability within these hydrogels are presented.
FAQs
The photoinitiator converts selected-wavelength light exposure into reactive species. Those species activate light-responsive polymer precursors, initiating polymerization and creating crosslinks between polymer chains. As crosslinks accumulate, the formulation develops a three-dimensional network, which accounts for the transition from a liquid formulation toward a gel with defined material structure.
Spatial control comes from exposing only chosen regions, while temporal control comes from deciding when exposure occurs. Together, these controls allow material formation to follow a desired geometry and timing rather than occurring throughout the formulation at once. This precision supports patterned constructs and helps establish defined shapes in bioengineering applications.
Crosslinking connects polymer precursors into a three-dimensional network, giving the resulting gel its solid or semi-solid character and defined properties. In tissue-engineering contexts, controlling this network helps create materials whose mechanical environment and structure more closely resemble those of biological tissues, supporting the design of scaffolds and other engineered constructs.
A basic workflow begins with a liquid polymer formulation containing light-responsive precursors and a photoinitiator. The formulation is arranged in the intended shape, then selected wavelengths are applied to generate reactive species and trigger polymerization and crosslinking. The resulting network forms the basis of a hydrogel, scaffold, or cell-laden construct.
The formulation must combine light-responsive polymer precursors with a photoinitiator so that exposure can produce the reactive species needed for crosslinking. Researchers also select an appropriate wavelength and control where and when light reaches the formulation. Coordinating these components and conditions determines how precisely the material forms its intended structure.
Bioengineering applications include fabricating hydrogels, tissue-engineering scaffolds, and cell-laden constructs with defined shapes and properties. The method also supports bioprinting and biomolecule encapsulation, where controlled material formation is valuable. These uses help researchers develop materials that more closely reproduce the structural and mechanical environment associated with biological tissues.