Mechanical Anisotropy

Mechanical anisotropy is the dependence of a material’s mechanical behavior on the direction of applied force, making it essential for understanding how natural and engineered structures bear loads. It arises when internal components, such as fibers, pores, crystals, or polymer chains, have an organized orientation that produces different stiffness, strength, or deformation along different axes. In bioengineering, mechanical anisotropy helps characterize tissues such as tendon, muscle, bone, and arterial walls, while guiding the design of biomaterials, tissue-engineering scaffolds, and prosthetic devices. Measuring and modeling directional behavior improves predictions of tissue function, implant performance, and mechanical compatibility with the body.

Mechanical Anisotropy - Related Videos

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JoVE EoE - Biomolecular Interaction Detection Techniques

Fluorescence Anisotropy-Based Detection of Protein-Protein Interactions

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2025

In this video, we describe the fluorescence anisotropy technique to study the interactions between the fluorophore-tagged Shwachman-diamond syndrome (SBDS) protein and the elongation factor-like 1 GTPase (EFL1). On incubating SBDS proteins with gradually increasing concentrations of EFL1, a steady increase in anisotropy is observed, indicating a successful interaction between the two proteins.

Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity

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2025

This video describes high-performance fluorescence anisotropy that helps to monitor the interaction between transcription factors and DNA. The assay monitors the interactions of a fluorophore-labeled DNA molecule with its specific transcription factor by measuring the degree of polarization due to molecular rotation or anisotropy of the fluorophore-labeled DNA.

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

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Cited by 37 •

2016

Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).

Mechanical Stimulation of Chondrocyte-agarose Hydrogels

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Cited by 29 •

2012

The biosynthesis of cartilaginous extracellular matrix by chondrocytes can be affected by application of mechanical stimuli. This method describes the technique of applying dynamic compressive strains to chondrocytes encapsulated in 3D constructs and the evaluation of induced changes in chondrocyte metabolism.

Research

JoVE Journal - Biochemistry
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Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

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Cited by 1 •

2025

Here, we present the protocol to study the local flexibility and dynamics of biomolecules using time-resolved fluorescence anisotropy at the single-molecule level in confocal microscopy mode.

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