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JoVE Journal
Biochemistry

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Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
 

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Article doi: 10.3791/54640
October 21st, 2016 Usage Statistics

Summary October 21st, 2016

Protein interactions are at the heart of a cell's function. Calorimetric and spectroscopic techniques are commonly used to characterize them. Here we describe fluorescence anisotropy as a tool to study the interaction between the protein mutated in the Shwachman-Diamond Syndrome (SBDS) and the Elongation factor-like 1 GTPase (EFL1).

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