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Chapters
- 00:05Title
- 01:22Isolation of Primary Articular Chondrocytes
- 04:07Chondrocyte-agarose Hydrogel Encapsulation
- 06:08Mechanical Stimulation and Radiolabeling of Chondrocyte-agarose Hydrogels
- 10:56Representative Assay Data
- 12:26Conclusion
The biosynthesis of cartilaginous extracellular matrix by chondrocytes can be affected by application of mechanical stimuli. This method describes the technique of applying dynamic compressive strains to chondrocytes encapsulated in 3D constructs and the evaluation of induced changes in chondrocyte metabolism.
Tags
Mechanical StimulationChondrocyteAgarose HydrogelsArticular CartilageRepair CapacityMechanical InsultOsteoarthritisBiochemical PropertiesDurabilityLong-term SurvivabilitySmall Focal DefectsMechanical PropertiesExtracellular Matrix SynthesisDynamic CompressionPhysiologic StrainsFrequencyChondrocyte MetabolismBiosynthesis
