Oil Phase Removal
Oil Phase Removal - Related Videos
11:20
In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
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Cited by 1 •
2017
This article describes a setup and method for the in situ visualization of oil samples under a variety of temperature and pressure conditions that aim to emulate refining and upgrading processes. It is primarily used for studying isotropic and anisotropic media involved in the fouling behavior of petroleum feeds.
13:19
Enhanced Oil Recovery using a Combination of Biosurfactants
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Cited by 2 •
2022
We illustrate the methods involved in screening and identification of the biosurfactant producing microbes. Methods for chromatographic characterization and chemical identification of the biosurfactants, determining the industrial applicability of the biosurfactant in enhancing residual oil recovery are also presented.
09:42
Solid Phase Synthesis
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2023
Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA Merrifield's solid-phase synthesis is a Nobel Prize winning invention where a reactant molecule is bound on a solid support and undergoes successive chemical reactions to form a desired compound. When the molecules are bound to a solid support, excess reagents and byproducts can be removed by washing away the impurities, while the target compound remains bound to the resin. Specifically, we will...
02:39
Phase Diagrams
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2020
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
02:31
Phase Transitions
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2020
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to occupy...
FAQs
Separation is driven primarily by the contrast between the two phases. Polarity limits mixing, density promotes formation of distinct layers, and interfacial behavior influences how readily the boundary can be disturbed or cleanly withdrawn. These factors determine whether the aqueous or biological fraction remains intact during oil phase removal, which is especially important when droplets contain cells or biomolecules.
The interface is the critical control point: withdrawing too close to it can transfer oil into the retained phase, whereas excessive distance can leave valuable aqueous material behind. Sample sensitivity also matters. Gentle handling helps maintain concentration, viability, and structural integrity, while unsuitable disturbance can compromise the material before downstream culture, purification, imaging, or analysis.
Aspiration and pipetting provide direct withdrawal after layers separate, while centrifugation can be used when phase separation needs compatible physical assistance. The appropriate choice depends on the sample and on how much interfacial disturbance can be tolerated. In each case, the goal is selective oil removal rather than simply maximizing the speed of layer separation.
Begin by allowing or achieving phase separation, then identify the oil boundary and choose a compatible withdrawal method. Remove the oil while keeping the aqueous or biological fraction as stationary as possible. If the sample contains encapsulated cells or biomolecules, retain the recovered fraction for the intended downstream step while minimizing additional handling.
Oil phase removal is useful when an emulsion or droplet system must yield an accessible aqueous or biological fraction. It can support recovery of aqueous contents, transfer of encapsulated cells or biomolecules, and preparation for downstream analysis, culture, purification, or imaging. The separation step therefore links droplet-based handling with subsequent biological processing.
Successful handling preserves the properties needed for the next experiment: biological viability, sample concentration, and structural integrity. Researchers should therefore consider whether the retained fraction was recovered without excessive disturbance and whether oil removal was sufficiently selective for the downstream task. These outcomes determine whether the sample is suitable for analysis, culture, purification, or imaging.