Surface Functionalization
Surface Functionalization - Related Videos
10:40
A Method of Targeted Cell Isolation via Glass Surface Functionalization
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Cited by 6 •
2016
This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.
09:12
Surface Functionalization of Hepatitis E Virus Nanoparticles Using Chemical Conjugation Methods
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Cited by 15 •
2018
We have engineered the capsid protein of hepatitis E virus as a theranostic nanoparticle (HEVNP). HEVNP self-assembles into a stable icosahedral cage in mucosal delivery. Here, we describe the modification of HEVNPs for tumor targeting by mutating surface-exposed residues to cysteines, which conjugate synthetic ligands that specifically bind tumor cells.
08:25
Combined Invasive Subcortical and Non-invasive Surface Neurophysiological Recordings for the Assessment of Cognitive and Emotional Functions in Humans
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Cited by 5 •
2016
The present protocol aims at assessing cognitive-emotional functions in the basal ganglia by simultaneous neurophysiological recording of local field potentials and non-invasive brain cortical activity (EEG). The procedure is exemplified by the use of paradigms involving speech stimuli with emotional connotation or the Flanker task involving cognitive control.
06:14
Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces
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Cited by 2 •
2018
Presented is an easy method to fabricate nano-micro multiscale structures, for functional surfaces, by aggregating nanofibers fabricated using an anodic aluminum oxide filter.
08:12
Surface Functionalization of Metal-Organic Frameworks for Improved Moisture Resistance
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Cited by 7 •
2018
Robust functional catechol coatings were produced in one step by direct reaction of the material known as HKUST with synthetic catechols under anaerobic conditions. The formation of homogeneous coatings surrounding the entire crystal is ascribed to the biomimetic catalytic activity of Cu(II) dimers on the external surface of the crystals.
FAQs
Surface functionalization can modify an interface by covalent bonding, adsorption, or other chemical and physical processes. Covalent attachment forms a chemical link to the surface, whereas adsorption places material at the interface without describing that same bonding route. This choice determines how molecules, polymers, coatings, or biomolecules are presented for subsequent interactions.
Changes in wettability, charge, and reactivity alter how the engineered surface interacts with its surroundings. Controlling these properties can influence protein binding, cell adhesion, immune responses, and biomaterial integration. Surface functionalization therefore provides a way to tune biological and chemical behavior without relying only on the material’s original outer-layer characteristics.
Ligand presentation controls how selected biomolecules are displayed at a surface, making it relevant to interactions with cells and proteins. Because engineered interfaces can regulate cell adhesion and protein binding, the identity and presentation of surface-associated biomolecules can affect how a scaffold, implant, drug-delivery system, or analytical device performs in a biological environment.
The desired outcome should determine which surface characteristics receive emphasis. A tissue-engineering scaffold or implant may prioritize cell adhesion, immune responses, or biomaterial integration, while a biosensor or microfluidic device may emphasize controlled chemical interactions and analytical sensitivity. Drug-delivery systems likewise require interface properties suited to their biological surroundings.
Common systems include tissue-engineering scaffolds, implant coatings, drug-delivery systems, microfluidic devices, and biosensors. In each case, modifying the interface helps address a different performance need, such as supporting cell attachment, improving compatibility, regulating biological interactions, or increasing the sensitivity of an analytical platform.
In biosensors and microfluidic devices, surface modification can control chemical reactivity, charge, wettability, and biomolecule presentation. These changes influence how the interface interacts with substances moving through or contacting the device. The resulting control can improve analytical sensitivity while helping the platform interact more selectively with its surrounding biological or chemical environment.