Vascularized Adipose Tissue
Vascularized Adipose Tissue - Related Videos
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
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Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
07:09
Isolation of Adipose Tissue Immune Cells
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Cited by 75 •
2013
Adipose tissue (AT) is a site of intense immune cell activation and interaction. Almost all cells of the immune system are present in AT and their ratios are altered by obesity. Proper isolation, quantification, and characterization of AT immune cell populations are critical for understanding their role in immunometabolic disease.
09:20
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Cited by 47 •
2016
This protocol describes the isolation of pig adipose-derived stem cells (pADSC) from subcutaneous adipose tissues with examination of multipotency. The multipotent pADSC are used to delineate processes of adipocyte differentiation and study transdifferentiation into multiple cell lineages of mesodermal mesenchyme or further lineages of ectoderm and endoderm for regenerative studies.
03:52
Isolating Adipose-Derived Stem Cells from Murine Periaortic Adipose Tissue
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2025
This video demonstrates a technique for isolating and culturing fluorescently labeled neural crest-derived adipose-derived stem cells (ADSCs) from murine periaortic arch adipose tissue. Following isolation, the NCADSCs are detected, sorted using fluorescence-activated cell sorting, and subsequently cultured on a plate, promoting cell proliferation.
07:24
Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy
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Cited by 4 •
2017
Videomicroscopy systems are used to examine functional properties of isolated adipose tissue arterioles in response to physiological and pharmacological stimuli. This technique can be used to examine microvascular phenotypes in different adipose tissue domains in obese humans.
FAQs
Functional vessels do more than occupy the graft: they deliver oxygen and nutrients, helping living adipose tissue remain viable after transplantation or fabrication. This support also preserves physiological activity and encourages integration with host circulation, making vascularization a central design goal in engineered fat constructs.
Vascularized adipose tissue can be engineered by combining adipocytes or adipose-derived stem cells with a biomaterial scaffold and pro-angiogenic signals. The scaffold provides a structured setting, while the signals encourage endothelial cell migration and capillary formation. Coordinating these elements links tissue formation to vessel development, which can improve oxygenation and nutrient delivery within the construct.
Adipocytes and adipose-derived stem cells supply the cellular basis for the engineered tissue, while endothelial cell behavior establishes the vascular component. In the design, these cells are paired with a biomaterial scaffold and pro-angiogenic signals rather than treated as isolated ingredients. Their coordinated use supports capillary formation and helps the resulting construct function as living adipose tissue.
A bioengineering workflow begins by selecting adipocytes or adipose-derived stem cells, then combining them with a biomaterial scaffold and pro-angiogenic signals. The resulting construct is designed to promote endothelial cell migration and capillary formation. Researchers then seek functional connection with host circulation, because that integration supports graft oxygenation, nutrient delivery, and survival after transplantation.
Applications span soft tissue reconstruction, adipose biology modeling, and studies of obesity or metabolic disease. In regenerative medicine, engineered vascularized fat may help address soft tissue defects while also providing a biologically relevant system for investigating adipose function and disease-related processes in bioengineering studies.
By combining living adipose cells with developing vascular networks, engineered constructs can provide more physiologically relevant platforms for testing therapies. They also allow researchers to study adipose biology and investigate obesity or metabolic disease in a controlled bioengineering context. The value lies in connecting tissue behavior with vascular support rather than examining adipose components in isolation.