Cell Index Monitoring
Cell Index Monitoring - Related Videos
13:38
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Cited by 21 •
2012
FRET-based reporters are increasingly used to monitor kinase and phosphatase activities in live cells. Here we describe a method on how to use FRET-based reporters to assess cell cycle-dependent changes in target phosphorylation.
08:17
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
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Cited by 12 •
2016
A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets.
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
13:48
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Cited by 20 •
2012
Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.
09:02
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
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Cited by 45 •
2013
This protocol describes the development, expansion, and in vivo imaging of NK cells derived from hESCs and iPSCs.
FAQs
Electrical resistance changes when adherent cells interact with microelectrodes beneath the culture area. As cells attach, spread, increase in number, or alter their morphology and barrier properties, they modify the measured signal. The resulting cell index therefore reflects combined changes in cell coverage and behavior rather than cell number alone, making curve interpretation dependent on the biological response being studied.
Cell number is only one contributor to a cell index curve. The trace may also shift as cells attach, spread, change morphology, or modify barrier properties. These signals help connect an electrical response with a cellular process, while reminding researchers that the curve represents composite behavior rather than an isolated measurement of one variable.
Cell Index Monitoring preserves the sequence of cellular responses instead of reducing an assay to a single observation. Continuous curves can show when proliferation, cytotoxicity, migration, or another response becomes apparent, including effects that endpoint measurements may miss. Because the culture is not repeatedly disturbed for each reading, the same assay can be followed dynamically across its response.
An experiment typically begins by growing adherent cells on a culture plate containing microelectrodes. The system then records electrical measurements continuously while the culture responds under assay conditions. Researchers examine the resulting cell index curve for time-dependent changes associated with growth, toxicity, migration, infection, or other cellular behavior, avoiding repeated handling of the same culture.
Drug screening and toxicology use the method to examine cellular responses over time, including effects associated with cytotoxicity. In infection studies and cell-based assays, continuous curves provide a dynamic record of changing cell behavior rather than only an endpoint result. This can reveal whether responses emerge during the observation period and support interpretation of time-dependent effects.
Infection studies can use continuous cell index measurements to follow changes in cellular behavior during the assay period. The resulting curves provide time-dependent information that may reveal growth-related changes, migration, cytotoxicity, or other responses associated with the experimental condition. This makes the technique useful when the timing of a cellular effect matters alongside its eventual outcome.