Dextran Calibration
Dextran Calibration - Related Videos
07:43
Calibration Curves
0 Views •
2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error...
01:11
Glassware Calibration
0 Views •
2024
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes. Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
0 Views •
2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
12:00
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
0 Views •
Cited by 9 •
2015
The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.
08:12
Pressure Transducer: Calibration Using a Pitot-static Tube
0 Views •
2023
Source: Shreyas Narsipur, Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC Fluid pressure is an important flow characteristic that is required to determine the aerodynamics of a system. One of the oldest and still existing pressure measurement systems is the manometer due to its accuracy and simplicity of operation. The manometer is generally a U-shaped glass tube that is partially filled with liquid, as shown in Figure 1. The U-tube manometer requires no...
FAQs
Dextran standards move through the pores of a gel or column, and each standard produces a measurable elution volume or retention time. These recorded values describe how the size-exclusion system handles molecules across the tested molecular-weight range. Comparing the standards therefore links chromatographic behavior with molecular size without relying only on an unknown sample's signal.
A calibration curve commonly relates elution behavior to the logarithm of dextran molecular weight rather than to molecular weight in an untransformed form. This provides the reference relationship used to interpret chromatographic measurements. Once the curve is established, an unknown sample's elution volume or retention time can be compared with that relationship to estimate molecular size.
The standards show which molecular-weight region the system can meaningfully separate and evaluate. Their elution behavior helps researchers assess the separation range of the porous gel or column and judge chromatographic performance across that range. This information is important when deciding whether the system is suitable for characterizing a particular protein, polymer, or other macromolecule.
A set of dextran reference points establishes how chromatographic behavior corresponds to molecular weight. Measurements from an unknown sample can then be interpreted against that reference, allowing estimation of molecular size and contributing to size-distribution analysis for macromolecules in solution. The resulting interpretation is grounded in the system's measured separation behavior rather than an isolated elution measurement.
First, dextran standards with known molecular weights are passed through the porous gel or column. The elution volume or retention time for each standard is recorded, and those measurements are used to construct a calibration curve, commonly against the logarithm of molecular weight. Unknown samples can then be evaluated by comparing their elution behavior with the curve.
The key recorded measurement is each dextran standard's elution volume or retention time as it passes through the size-exclusion system. These values become the chromatographic coordinates of the calibration data. When paired with the standards' known molecular weights, they provide the reference points needed to estimate the molecular size of unknown samples.
Researchers use this approach when they need to characterize chromatographic performance, evaluate a system's separation range, or estimate the size of macromolecules in solution. It can support analysis of proteins, polymers, and other molecular species whose chromatographic behavior can be compared with dextran standards. The method is therefore useful for interpreting size-exclusion results in biological laboratory workflows.
After calibration, an unknown protein, polymer, or other macromolecule is analyzed under the size-exclusion system and its elution behavior is recorded. That result is located against the dextran-based calibration relationship to estimate molecular size. The comparison also helps place the unknown within the system's evaluated separation range, supporting more informed interpretation of its chromatographic result.