Scan Depth Adjustment

Scan depth adjustment is the control of how deeply an imaging system focuses or collects optical information within a biological sample, helping researchers select the tissue region relevant to their experiment. In depth-resolved microscopy, the focal plane is shifted through the specimen while image quality changes with light scattering, absorption, refractive-index differences, and signal attenuation; selecting an appropriate depth balances penetration with resolution and contrast. This adjustment supports optical sectioning, three-dimensional reconstruction, and analysis of cells or structures located at different positions in tissue, improving the accuracy and interpretability of biological imaging data.

Scan Depth Adjustment - Related Videos

Research

JoVE Journal - Engineering

In Depth Analyses of LEDs by a Combination of X-ray Computed Tomography (CT) and Light Microscopy (LM) Correlated with Scanning Electron Microscopy (SEM)

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Cited by 2 •

2016

A workflow for comprehensive micro-characterization of active optical devices is outlined. It contains structural as well as functional investigations by means of CT, LM and SEM. The method is demonstrated for a white LED which can be still be operated during characterization.

Education

JoVE Science Education - Chemistry

Scanning Electron Microscopy (SEM)

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2023

Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...

The Anchoring-and-Adjustment Heuristic

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2020

In order to make good decisions, we use our knowledge and our reasoning. Often, this knowledge and reasoning is sound and solid. However, sometimes, we are swayed by biases or by others manipulating a situation. For example, let’s say you and three friends wanted to rent a house and had a combined target budget of $1,600. The realtor shows you only very run-down houses for $1,600 and then shows you a very nice house for $2,000. Might you ask each person to pay more in rent to get the $2,000...

Adjusting a Traverse

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2025

In the site survey of a four-sided traverse, internal angles are essential to ensure geometric accuracy. The survey revealed that the sum of the measured internal angles was 359 degrees and 48 minutes, which is 12 minutes less than the expected 360 degrees. This discrepancy signals an error likely arising from measurement inaccuracies during the fieldwork.To rectify this error, the adjustment process involved distributing the 12-minute shortfall equally across the four internal angles. By...

Adjusting Entries

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2025

In accounting, a business's economic activities are segmented into designated time intervals, typically monthly, quarterly, or annually, known as accounting periods. This segmentation facilitates consistent tracking, summarization, and reporting of financial data, enabling stakeholders to accurately evaluate a company's performance and financial position. Companies must incorporate adjusting entries at the close of each period to ensure that financial reports conform to the accrual basis of...

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