Serum Antibody Profiling
Serum Antibody Profiling - Related Videos
12:19
Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
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Cited by 146 •
2009
The technical aspects of performing LIPS (Luciferase Immunoprecipitation Systems) are described. The overall approach involves expressing chimeric genes encoding antigens fused to Renilla luciferase (Ruc) in mammalian cells. Crude Ruc-antigen extracts are then prepared and, without purification, employed in immunoprecipitation assays to quantify antibodies.
05:33
A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
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2025
This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...
03:59
The Hemagglutination Inhibition Assay to Detect Serum Antibodies Against a Target Antigen
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2025
The video demonstrates a hemagglutination inhibition assay for detecting serum antibodies against specific antigens. Confirmation of hemagglutination inhibition in the test well and hemagglutination in the control well is achieved through distinct visual patterns. This assay plays a crucial role in assessing immune response, vaccine effectiveness, and studying viral infections in virology and immunology.
05:21
A Peptide Microarray Technology for Specificity Profiling of Antibodies
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2025
This video demonstrates a technique that applies peptide microarray technology to profile the specificity of antibodies recognizing post-translational modifications (PTMs) on histone peptides. A microarray slide containing immobilized fluorophore-conjugated histone peptides with known PTM combinations is treated with target PTM-specific antibodies. This process helps identify the antibody's specificity for the target and its potential cross-reactivity with non-target PTMs.
15:17
Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Cited by 19 •
2011
We described a procedure for the disaggregation of colorectal cancer (CRC) to produce viable single cells, which are then captured on customized antibody microarrays recognizing surface antigens (DotScan CRC microarray). Sub-populations of cells bound to the microarray can be profiled by fluorescence multiplexing using monoclonal antibodies tagged with fluorescent dyes.
FAQs
Signal intensity is interpreted as a measure of binding strength or recognition pattern, not as a complete immune summary. Comparing signals across selected antigens can reveal which targets are recognized and how strongly, while examining the full pattern helps describe antibody diversity. Thus, abundance, specificity, and diversity provide complementary views of humoral activity in the sample.
Antigen selection determines what the resulting profile can reveal. A panel containing different selected antigens allows the assay to compare recognition across targets, whereas a narrow panel provides a more focused view. Multiplex formats extend this comparison within one analytical platform, making the pattern of binding central to interpretation rather than relying on a single readout.
Distinguishing antibody classes or subclasses adds an additional layer to serum antibody profiling. Instead of considering only whether serum binds an antigen, the analysis can separate signals according to the antibody category measured. This refinement helps describe the composition of the humoral response and can identify differences that an aggregated antibody signal would conceal.
A basic workflow begins with serum analysis against selected antigens, followed by measurement of binding through an immunoassay or multiplex platform. The resulting signal intensities or related readouts are then organized by antigen and, when included, by antibody class or subclass. Researchers interpret the combined pattern to assess recognition, abundance, and diversity.
Immunoassays and multiplex platforms answer related questions but differ in how broadly recognition is examined. An immunoassay can measure binding to selected antigens, while multiplexing supports simultaneous assessment across a set of targets. The choice affects the breadth of the resulting profile and should match whether the study prioritizes focused or comparative antigen analysis.
In biological techniques research, these profiles can be applied to infection and vaccination studies, autoimmune and immune-mediated disorders, biomarker discovery, and evaluation of therapeutic or diagnostic strategies. Their value lies in connecting molecular antigen recognition with broader immune activity. Interpreting the pattern across targets, rather than a single signal, can support comparisons among these research contexts.