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JoVE Journal
Biology

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Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
 

Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)

Article DOI: 10.3791/1549
October 7th, 2009

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Summary October 7th, 2009

The technical aspects of performing LIPS (Luciferase Immunoprecipitation Systems) are described. The overall approach involves expressing chimeric genes encoding antigens fused to Renilla luciferase (Ruc) in mammalian cells. Crude Ruc-antigen extracts are then prepared and, without purification, employed in immunoprecipitation assays to quantify antibodies.

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