Affordable Lighting System

An affordable lighting system is a low-cost arrangement of light sources, controls, and supports that provides reliable illumination for biological observation, cultivation, or experimentation. In biology, light-emitting diodes (LEDs) can deliver selected wavelengths and intensities, while timers regulate exposure duration and reduce heat and energy use compared with some conventional sources. These systems support plant growth studies, classroom demonstrations, microscopy, and laboratory work where consistent lighting is essential but resources are limited. By improving access to controlled illumination, affordable lighting systems can make biological research and education more reproducible, adaptable, and widely available.

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Research

JoVE Journal - Neuroscience

Using Affordable LED Arrays for Photo-Stimulation of Neurons

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Cited by 7 •

2011

Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.

Research

JoVE Journal - Bioengineering
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Simple, Affordable, and Modular Patterning of Cells using DNA

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Cited by 11 •

2021

Here we present a protocol to micropattern cells at single-cell resolution using DNA-programmed adhesion. This protocol uses a benchtop photolithography platform to create patterns of DNA oligonucleotides on a glass slide and then labels cell membranes with commercially available complementary oligonucleotides. Hybridization of the oligos results in programmed cell adhesion.

Construction of an Affordable and Easy-to-Build Zebrafish Facility

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Cited by 13 •

2014

A Zebrafish facility suitable for breeding and in vivo research is built in an easy and affordable manner. Maintenance is minimal. This facility is ideal for student involvement and independent research.

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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Cited by 11 •

2022

The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

Rapid, Affordable, and Uncomplicated Production of Bacterial Cell-free Lysate

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Cited by 3 •

2021

This protocol describes a rapid and simple method to produce bacterial lysate for cell-free gene expression, using an engineered strain of Escherichia coli and requiring only standard laboratory equipment.

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