Tat-pip
Tat-pip - Related Videos
01:42
Phosphoinositides and PIPs
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2023
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs. Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10:58
PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
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Cited by 7 •
2017
Here we present a supported lipid bilayer in the context of a microfluidic platform to study protein-phosphoinositide interactions using a label-free method based on pH modulation.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
10:30
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Cited by 10 •
2015
We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.
09:40
Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Cited by 2 •
2011
Here, we describe detailed live cell imaging methods for investigating chemotaxis. We present fluorescence microscopic methods to monitor spatiotemporal dynamics of signaling events in migrating cells. Measurement of signaling events permits us to further understand how a GPCR-signaling network achieves gradient sensing of chemoattractants and controls directional migration of eukaryotic cells.
FAQs
Cell entry depends on the Tat segment’s positive charge. It associates with negatively charged components at the cell surface and promotes uptake, often through endocytosis, bringing the attached PIP into the cell. This transport step matters because the interaction sequence must reach the intracellular environment before it can engage its molecular target.
The PIP sequence determines the intended intracellular molecular target and the protein interaction it is designed to recognize. Once the Tat segment has promoted entry, that sequence can engage the target and disrupt or modify the associated interaction. Thus, Tat-PIP activity reflects a delivery function joined to target-directed molecular action.
A Tat segment can promote cellular uptake, but uptake alone does not explain how the construct affects biology. The PIP supplies the sequence intended to bind a specific intracellular target and disrupt or modify a protein interaction. Considering both components links cell entry with the molecular change under investigation.
In cultured cells, investigators use them as experimental tools to bring a target-directed peptide into the intracellular setting. They can then examine signaling pathways, assess consequences of regulating a protein’s function, or test a peptide-based inhibitor concept. The approach connects peptide design with cellular biology rather than limiting analysis to isolated interactions.
Their applications include probing signaling pathways and examining how changing a protein interaction influences cellular biology. Because the PIP is designed for a specific molecular target, researchers can use different sequences as tools for target-directed regulation or for testing peptide-based inhibitors in cultured cells directly.
Their ability to reach intracellular targets gives Tat-PIPs value beyond pathway analysis. The same delivery and target-engagement features can be used to explore peptide-based strategies intended to disrupt or modify specific protein interactions, with the overview framing this use as experimental therapeutic development rather than an established treatment.