Aggregate Formation Protocol
Aggregate Formation Protocol - Related Videos
04:12
Protein Aggregate Formation Assay: A Method to Detect and Quantify Protein Aggregation in Cultured Cells upon Induction by Proteasome Inhibitor
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2025
In this video, we demonstrate a cell-based protein aggregation assay using proteasome inhibitors, which block proteasome activity, preventing misfolded, mutant proteins, fused to a fluorescent label, from undergoing ubiquitin-dependent proteasomal degradation, leading to their accumulation within the cell cytoplasm. The protein aggregates are then visualized and quantified by fluorescence microscopy.
07:19
Protocol for Biofilm Streamer Formation in a Microfluidic Device with Micro-pillars
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Cited by 17 •
2014
Protocols for the study of biofilm formation in a microfluidic device that mimics porous media are discussed. The microfluidic device consists of an array of micro-pillars and biofilm formation by Pseudomonas fluorescens in this device is investigated.
10:10
Aggregates for Concrete and Asphaltic Mixes
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2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA Concrete and asphalt are by far the most common construction materials used today. Concrete is a composite material consisting of cement, water, air, coarse aggregate, and fine aggregates. Fine aggregates are typically sands and coarse aggregates are natural or crushed rocks. Chemical admixtures to modify certain specific properties are also commonly used (i.e., superplasticizers to make the...
10:10
Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
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Cited by 29 •
2009
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
07:46
An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production
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Cited by 67 •
2017
Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.
FAQs
Individual cells first come into close contact under nonadherent conditions. Intercellular adhesion then holds neighboring cells together, while compaction reduces the space between them and promotes a more organized three-dimensional structure. These processes are important because they help produce stable aggregates that can model tissue-like interactions rather than remaining as loosely associated cells.
Nonadherent conditions prevent cells from attaching primarily to a culture surface, encouraging them to interact with one another instead. This shifts the system toward cell-cell organization, allowing adhesion and compaction to drive cluster formation. The resulting arrangement is useful when researchers need a three-dimensional model of cellular behavior rather than a conventional two-dimensional culture.
Aggregate size and cell viability influence how consistently experimental models form and perform. Similar aggregate dimensions support more comparable tissue-like organization across samples, while adequate viability helps preserve the cellular interactions being studied. Monitoring these outcomes is therefore central to reproducibility in applications such as differentiation studies, organoid generation, and drug-response assays.
A typical workflow begins with individual cells and places them under nonadherent conditions that promote close cell-cell contact. The cells then undergo adhesion and compaction as they assemble into a stable cluster. Researchers assess whether aggregates form consistently, including their size and viability, before using them for downstream biology, tissue engineering, or assay applications.
Researchers may choose aggregates when cell-cell organization and tissue-like interactions are important to the question being studied. Compared with many two-dimensional cultures, three-dimensional aggregates provide a more physiologically relevant context for examining cell behavior, differentiation, development, or disease. They can also serve as controlled starting material for later organoid or tissue-engineering work.
Once stable aggregates have formed, they can provide starting material for organoid generation and tissue-engineering approaches. Their three-dimensional organization also supports drug-response assays in a context that reflects cell-cell interactions. Consistent formation, size, and viability help researchers compare responses across samples and interpret whether observed effects relate to the tested condition.