Sciatic Nerve Transection
Sciatic Nerve Transection - Related Videos
05:29
Axoplasm Isolation from Rat Sciatic Nerve
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Cited by 6 •
2010
We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.
03:51
Murine Sciatic Nerve Model of Perineural Invasion or PNI: Extraction and Processing of Sciatic Nerve to Understand Invasion by Cancer Cells
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2025
In this video, we describe the procedure to establish a murine model of perineural invasion by injecting cancer cells into the sciatic nerve. The model can help gain an understanding of the magnitude of nerve invasion for subsequent assessment of anti-cancer drugs.
10:50
Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
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Cited by 38 •
2013
The tibial nerve transection model is a well-tolerated, validated, and reproducible model of skeletal muscle atrophy. The model surgical protocol is described and demonstrated in C57Black6 mice.
02:40
Axonal Regeneration in the Sciatic Nerve of a Mouse Following a Crush Injury
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2025
Source: Li, Q., et al. Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion. J. Vis. Exp. (2018)This video demonstrates the process of axonal regeneration in a mouse sciatic nerve following a crush injury. The isolated nerve is fixed and prepared for imaging, with fluorescent proteins expressed in neurons to visualize axon growth. Using an epifluorescence microscope, axon regeneration is traced from the injury site to assess the extent of...
07:09
The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice
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Cited by 78 •
2014
Neuropathic pain is a consequence of a lesion or disease affecting the somatosensory system. The “cuff model” of neuropathic pain in mice consists of the implantation of a polyethylene cuff around the main branch of the sciatic nerve. Mechanical allodynia is tested using von Frey filaments.
FAQs
The immediate biological consequence is loss of axonal continuity across the injury site. The distal nerve segment, separated from the neuronal cell body, undergoes Wallerian degeneration, while signals can no longer pass normally between the spinal cord and hind-limb targets. This creates a defined injury state for studying subsequent repair.
Denervation interrupts communication between the spinal cord and affected hind-limb muscles and sensory targets. As a result, the injured system provides a way to examine how loss of neural input relates to disrupted motor and sensory function. These changes are central to evaluating whether later repair restores communication and tissue function.
A complete surgical separation establishes a clearly defined interruption of the nerve pathway. Researchers can then follow biological responses associated with peripheral nerve injury, including Wallerian degeneration, axonal regeneration, and neuroinflammation. Because the injury is well delimited, the model supports assessment of how repair progresses after axonal continuity has been lost.
This model supports investigation of several linked responses to nerve injury rather than only the initial loss of function. Studies can examine degeneration in the distal segment, inflammatory responses around the injury, attempts at axonal regeneration, and the eventual relationship between structural repair and functional recovery in the hind limb.
Researchers can assess tissue repair, reinnervation, and restoration of motor or sensory function. Tissue repair reflects changes at the injury site, whereas reinnervation indicates renewed neural connection with target tissues. Motor and sensory measurements provide functional outcomes, helping determine whether biological repair translates into recovery of hind-limb performance.
After establishing the injury, investigators can use the model to test nerve grafts, biomaterials, or other regenerative therapies. Their performance can be compared through evidence of tissue repair, axonal reinnervation, and recovered motor or sensory function. These outcomes help connect the material or treatment response with biological restoration after nerve damage.