Arl13b-centrin-2 Mice
Arl13b-centrin-2 Mice - Related Videos
09:53
Volumetric Imaging and Analysis of Primary Cilia in Musculoskeletal Tissue using the ARL13B-CENTRIN-2 Mouse Model
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Cited by 1 •
2025
This method describes the exploitation of the endogenous signal in an ARL13B-CENTRIN-2 transgenic mouse model to image primary cilia in situ, in three dimensions across large areas of mineralized tissue, from tissue collection to analysis of cilia organization. This protocol may also be applied to other types of tissues.
08:10
In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
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Cited by 28 •
2008
To understand network dynamics of microcircuits in the neocortex, it is essential to simultaneously record the activity of a large number of neurons . In-vivo two-photon calcium imaging is the only method that allows one to record the activity of a dense neuronal population with single-cell resolution .
16:48
Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
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Cited by 20 •
2007
Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph node.
03:17
Static Strength Training Method for Type 2 Diabetic Mice
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Cited by 1 •
2024
This protocol provides a simple method of making static training equipment for mice. The device maintains the muscle isometric contraction of the limbs of mice so as to verify the intervention effect of traditional exercise on type 2 diabetes (T2DM) and provides new exercise therapy for the clinical treatment of T2DM.
07:37
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
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Cited by 5 •
2018
We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.
FAQs
The Arl13b-based reporter follows the ciliary membrane, whereas the centrin-2 reporter marks centrioles and centrosomes. Their different cellular localizations allow researchers to identify these structures as separate fluorescence signals within the same tissue. This distinction supports analysis of how cilia and centrosomes are positioned, assembled, and maintained in living biological settings.
Simultaneous labeling connects cilium behavior with the organization of its associated centrosomal structures. Researchers can examine whether changes in centriole or centrosome position accompany differences in cilium formation or dynamics. This paired view is especially useful when studying cellular organization, division, or tissue development, where both structures may change over time.
The model supports examination of cilium formation, centrosome organization, and cell division through observable fluorescence patterns. Because these structures can be followed in living tissues, researchers can relate their assembly and positions to broader developmental changes. The approach therefore connects subcellular organization with tissue-level biology rather than limiting analysis to isolated cellular structures.
Perturbations can be evaluated by examining changes in the location, assembly, or dynamics of the labeled cilia and centrosomes. A difference in fluorescence patterns provides a direct cellular readout of how an altered gene or environmental condition affects these structures. This makes the model useful for linking experimental changes to biological organization in intact tissues.
Fluorescence microscopy converts the reporters' locations into signals that can be observed directly within intact organisms. Researchers can therefore examine where cilia and centrosomes occur and how their organization changes without relying only on indirect measurements. The resulting observations help connect cellular structures with tissue development and ongoing biological activity.
The model is particularly relevant to developing neural and sensory systems, where cilia and centrosomes contribute to tissue organization. Researchers can use the fluorescent signals to investigate how these structures are positioned and assembled during development in those tissues. Such observations provide cellular context for changes that may influence neural or sensory biology.
Arl13b-centrin-2 mice provide visible cellular markers for examining abnormalities in cilia and related centrosomal structures. Researchers can assess how genetic changes or environmental perturbations alter their formation, position, or dynamics in tissues. These observations help connect structural defects with affected biological systems and support investigation of the cellular basis of ciliary disorders.