Auditory Bulla Dissection
Auditory Bulla Dissection - Related Videos
07:40
Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
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Cited by 13 •
2017
We present a protocol to isolate the auditory bulla, capsule, and ossicles from postnatal mice for whole mount and histological analysis.
02:43
Recording Electrically-Evoked Auditory Brainstem Responses in a Deafened Mouse
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2025
This video demonstrates the procedure to implant a cochlear device in a deafened mouse and apply electrical stimuli to record auditory brainstem responses. This procedure enables to understand the basic mechanisms of electrical hearing and can contribute to advancements in cochlear implant technology and auditory neuroscience.
10:14
In Ovo Electroporation in the Chicken Auditory Brainstem
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Cited by 6 •
2017
Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development.
11:16
Preparation and Culture of Chicken Auditory Brainstem Slices
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Cited by 17 •
2011
The chicken auditory brainstem is comprised of nuclei responsible for binaural sound processing. A single coronal slice preparation maintains the entire circuitry while the cultured approach provides a unique preparation to study the development of neuronal structure and auditory function at the molecular, cellular and network levels.
02:22
Measuring the Auditory Brainstem Response in Mice
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2025
Source: Lee, D., et. al. Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage. J. Vis. Exp. (2023)This video demonstrates the measurement of auditory brainstem response (ABR) in a mouse. Electrodes are placed on the head, behind the ear, and near the tail of an anesthetized mouse. Acoustic stimuli at various intensities are delivered, and electric signals generated are recorded as waveforms.
FAQs
Microscopic visualization helps the investigator expose delicate middle-ear structures while maintaining a clear view of their arrangement. This is especially important when examining the tympanic membrane, auditory ossicles, cochlear region, and associated tissues together. The resulting anatomical detail supports more precise comparisons among normal ears, disease models, injury, and treatment-related changes.
Preserving spatial relationships allows researchers to interpret each structure within its anatomical context rather than as an isolated tissue sample. The position of the tympanic membrane, ossicles, cochlear region, and nearby tissues can therefore be considered together. This context helps connect structural changes with hearing biology, pathology, or developmental processes.
Removing surrounding temporal bone provides access to the air-filled middle-ear cavity and the structures enclosed within it. Careful bone removal exposes the region needed for observation, tissue collection, or later analysis while aiming to retain the relevant anatomical arrangement. This access is what makes detailed examination of otherwise hidden ear structures possible.
The general workflow involves using microscopic observation, removing surrounding temporal bone, and opening the bulla to expose the middle-ear region. Investigators then examine the tympanic membrane, auditory ossicles, cochlear region, and associated tissues, with tissue collection or preparation for histological analysis when required. The exact emphasis depends on the biological question.
Researchers use the approach to investigate ear development, middle-ear infection, trauma, ototoxicity, and hearing-related pathology. It is also suitable for examining how experimental treatments or devices affect the ear. By exposing the relevant anatomy directly, the technique links visible tissue changes with broader questions about disease mechanisms and auditory function.
Auditory Bulla Dissection can provide direct anatomical observations, access to tissues for collection, and material for histological analysis. These outcomes support evaluation of structural changes associated with infection, trauma, ototoxicity, development, or other hearing-related conditions. The exposed region can also be used to assess experimental treatments or devices in relation to ear anatomy.