Automated Gel Extraction
Automated Gel Extraction - Related Videos
03:22
SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion
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2023
In this video, we demonstrate the SDS-PAGE in-gel digestion method for protein extraction from extracellular vesicles or EVs. Once isolated, the obtained protein fragments can be used for proteomic analysis.
08:31
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Cited by 2 •
2013
Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.
13:26
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
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Cited by 9 •
2015
This manuscript describes an automated gel size selection approach for purifying DNA fragments for next-generation sequencing. The Ranger Technology provides complete automation of the entire process of agarose gel loading, electrophoretic analysis, and recovery of targeted DNA fragments allowing for high-throughput and high quality next-generation sequencing libraries.
10:47
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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Cited by 13 •
2013
TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.
06:30
Gel Purification
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2023
Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...
FAQs
The imaging system determines which gel band enters the extraction workflow, linking visual identification to size selection. Once the target is located, robotic excision removes the selected agarose region rather than relying on repeated manual cutting. This matters because the chosen band defines the DNA population recovered, while automated handling supports consistent processing and sample traceability.
After excision, the agarose matrix must be dissolved before DNA purification can proceed. The workflow may then use either silica-membrane binding or magnetic-bead binding, followed by washing and elution. These are alternative purification formats within the same automated sequence, and both are intended to produce purified DNA from the selected band for downstream molecular work.
Automation affects more than speed. Integrating imaging, excision, matrix dissolution, and purification reduces hands-on transfers and repeated manipulation, which can lower contamination risks and improve reproducibility. Consistent execution also strengthens sample traceability, allowing the processing history of recovered DNA to be followed more reliably across a set of biological samples.
An automated run proceeds as a linked series: identify the desired band by imaging, excise the corresponding gel, dissolve the agarose, bind the DNA to a silica membrane or magnetic beads, wash away unwanted material, and elute the purified nucleic acid. Keeping these stages integrated minimizes manual intervention and provides a repeatable path from gel band to recovered sample.
This workflow is useful when a biological experiment requires a clean, size-selected DNA fragment for cloning, sequencing, PCR product recovery, or another molecular application. The automated format is particularly relevant when multiple samples need consistent processing, clear traceability, and reduced hands-on manipulation during preparation of the nucleic acids.
The resulting product is purified DNA corresponding to the selected gel size, prepared for downstream molecular applications. In biology, this connects gel-based fragment selection with cloning, sequencing, and PCR product recovery. Workflow performance can also be assessed through processing speed, consistency, reproducibility, and traceability, while the recovered material supplies the size-selected nucleic acid.