Bacterial Vesicles
Bacterial Vesicles - Related Videos
03:24
Isolation of Bacterial Extracellular Vesicles Using Size-Exclusion Chromatography
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2025
Source: Watson, D. C., et al. Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria. J. Vis. Exp. (2021)This video demonstrates the use of size exclusion chromatography with porous resin beads to isolate bacterial extracellular vesicles (EVs) from a bacterial culture filtrate. Larger EVs elute early by bypassing the resin pores, while smaller proteins enter the pores and elute later.
04:51
Size Exclusion Chromatography Based Separation of Bacterial Extracellular Vesicles: A Technique to Separate Heterogenous Population of Gram-Negative Bacterial Outer Membrane Vesicles Based on Their Size
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2025
In this video, we demonstrate a technique–size exclusion chromatography–to separate a heterogenous population of outer membrane vesicles or OMVs derived from bacteria. These vesicles are nanosized spherical structures and are separated into large and small subpopulations based on their size through Size Exclusion Chromatography.
02:35
Single-Cell Culture of Bacteria Within Giant Vesicles
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2025
Source: Morita, M., et al. Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles. J. Vis. Exp. (2019).This video demonstrates a method for culturing and observing individual bacterial cells encapsulated inside giant vesicles anchored to a supported lipid bilayer. The system enables stable, long-term imaging of single-cell growth dynamics in a confined, physiologically relevant microenvironment.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
07:33
Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles
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Cited by 1 •
2019
We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells.
FAQs
Formation depends on the bacterial envelope. In Gram-negative bacteria, localized changes in envelope structure can make the outer membrane bulge and pinch off, producing outer membrane vesicles. Gram-positive bacteria have a thick cell wall, yet they can also release vesicles through that structure. These contrasting release routes connect vesicle production to major differences in bacterial cell architecture.
Bacterial vesicles can carry proteins, lipids, metabolites, and nucleic acids. Because these cargo classes represent different types of biological information and activity, a single vesicle population can influence several processes after delivery. Transporting this material beyond the bacterial cell enables effects on neighboring microbes as well as on host cells.
Vesicle-mediated delivery can shape biofilms, microbial competition, immune responses, and infection. Effects on neighboring bacteria may alter relationships within a microbial community, while delivery to host cells can influence interactions between microbes and their hosts. This range of outcomes makes vesicles relevant to both community biology and disease-related research.
Vesicles provide a biological route for bacteria to transport molecules beyond their own cell boundaries. Studying the cargo they carry and the recipients they influence can clarify how bacteria affect neighboring microbes and host cells. This supports investigation of microbial communication as a process involving directed movement of biological material through the surrounding environment.
Bacterial vesicles are studied in disease mechanisms, vaccine design, diagnostics, and engineered delivery systems. In disease research, they help examine how bacterial products influence hosts. In applied research, their transport capacity provides a basis for exploring diagnostic uses, vaccine-related strategies, and systems designed to deliver biological molecules.
Their relevance comes from the ability to deliver bacterial cargo to host cells and influence immune responses and infection. This creates a link between bacterial envelope biology and host outcomes. Examining that link can help researchers investigate disease mechanisms while also informing vaccine design and other approaches that depend on understanding bacterial interactions with hosts.