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Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

DOI:
10.3791/56440-v

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11:00 min

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November 09, 2017

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1BioLegend

Chapters

  • 00:05Title
  • 00:58Reagent and Standard Preparation
  • 02:57Assay Procedure
  • 06:23Flow Cytometer Set-up
  • 09:24Results: Quantification of Cytokines Produced by Mouse Splenocytes Cultured Under Stimulating Conditions
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.

Tags

Keywords: Multiplex Cytokine ProfilingMouse SplenocytesCytometric Bead-based ImmunoassayFlow CytometryCytokine QuantificationImmunologyELISAAntibody-immobilized BeadsMouse Th Cytokine StandardSerial DilutionAssay BufferFilter PlatesWash Buffer

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