Biotin-labeled Mrna
Biotin-labeled Mrna - Related Videos
04:10
Helicase Activity Measurement of a Target Protein Using Biotin-Labeled RNA Duplexes
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2025
In this video, we demonstrate the procedure to determine the helicase activity of a target protein to unwind the biotin-labeled dsRNA substrate. The activity of the enzyme was identified by analyzing the electrophoretic mobility shift, followed by a chemiluminescence assay using chemiluminescent enzyme-conjugated streptavidin.
11:00
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Cited by 31 •
2018
This report describes a fast and reliable method for validating mRNA targets of cellular miRNAs. The method uses synthetic biotinylated Locked Nucleic Acid (LNA)-based miRNA mimics to capture target mRNA. Subsequently, streptavidin-coated magnetic beads are employed to pulldown the target mRNA for quantification by qPCR polymerase chain reaction.
03:12
An Avidin-Biotin Conjugation Technique for Presenting Target Antigens on Mycobacterium bovis BCG
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2025
The video demonstrates a technique for loading antigens on Mycobacterium bovis BCG to improve its immunogenic properties. The method uses the avidin-biotin system to coat the bacterial surface with exogenous antigens.
08:33
Cell-Free In Vitro mRNA Translation In Drosophila Lysates
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2026
Here, we provide details on how to prepare lysates and perform in vitro translation using Drosophila embryos.
08:14
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
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Cited by 10 •
2019
Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions.
FAQs
The biotin moiety on the transcript binds strongly to streptavidin or avidin. When these binding partners are associated with a coated surface, the labeled mRNA can be retained for isolation. Alternatively, labeled detection reagents can reveal where the transcript is present, linking the RNA sequence to a detectable experimental signal.
Covalent attachment keeps the biotin tag associated with the mRNA while preserving the transcript sequence for subsequent analysis. This combination allows researchers to use the same RNA molecule for capture or visualization and then examine its identity or behavior downstream, rather than relying on a label that is merely associated with the transcript.
The intended experimental readout determines how the label is used. Binding to streptavidin- or avidin-associated surfaces supports transcript capture and isolation, whereas labeled detection reagents support visualization. Tracking applications focus on following the transcript in cells or cell-free systems, connecting its presence with distribution, interactions, or activity.
Detection of the biotin tag provides a way to follow a specific transcript while retaining information encoded by its mRNA sequence. In cellular studies, this supports analysis of RNA localization, allowing researchers to relate where a transcript is found to questions about gene expression, regulation, and cellular function.
A general workflow introduces the labeled transcript into a cellular or cell-free system, uses streptavidin or avidin binding when capture is needed, and applies detection reagents when visualization is the goal. The resulting material or signal can then support downstream analysis of transcript identity, localization, molecular interactions, or translation.
This approach supports studies of gene expression, RNA localization, molecular interactions, and translation. Researchers can examine these questions in cells or cell-free systems, depending on the experimental context. The resulting observations help connect a transcript with its distribution and function, providing context for research on development, regulation, and disease mechanisms.