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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
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JoVE Journal Genetics
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

DOI:
10.3791/59830-v

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08:14 min

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July 17, 2019

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, , , , , , , , , , , , ,
1State Key Laboratory of Reproductive Medicine,Nanjing Medical University, 2The Affiliated Hospital of Hangzhou Normal University, 3School of Life Science and Technology,ShanghaiTech University, 4Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease,Wuhan University

Chapters

  • 00:04Title
  • 00:40Protein Preparation
  • 03:25Nucleic Acid Preparation and In Vitro Biochemical Assay
  • 05:22Chemiluminescence Detection
  • 06:18Results: Interaction of MEIOB and MOV10 Proteins with Nucleic Acids
  • 07:49Conclusion

Summary

Automatic Translation

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Presented here are protocols for in vitro biochemical assays using biotin labels that may be widely applicable for studying protein-nucleic acid interactions.

Tags

Keywords: Biotin LabelsProtein-nucleic Acid InteractionsIn Vitro Biochemical AssaysMEIOBMOV10Affinity PurificationGST-tagged ProteinsFLAG-tagged ProteinsCentrifugationCell LysisMagnetic BeadsElution

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