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JoVE Journal
Immunology and Infection

This content is Open Access.

Legionella pneumophila Outer Membrane Vesicles

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Article DOI: 10.3791/55146
February 22nd, 2017


Summary February 22nd, 2017

Here, we describe the purification of Legionella pneumophila (L. pneumophila) outer membrane vesicles (OMVs) from liquid cultures. These purified vesicles are then used for the treatment of macrophages to analyze their pro-inflammatory potential.


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