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RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells

DOI:
10.3791/54044-v

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10:42 min

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June 04, 2016

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, ,
1The Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences,Tel Aviv University

Chapters

  • 00:05Title
  • 00:41Cell Preparation and Bacterial Infection
  • 04:26Nucleic Acid Extraction
  • 07:08DNase Treatment
  • 09:09Results: Listeria monocytogenes RNA Purification from Macrophage Cells
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method for bacterial RNA isolation from Listeria monocytogenes bacteria growing inside murine macrophages. This technique can be used with other intracellular pathogens and mammalian host cells.

Tags

Keywords: RNA PurificationListeria MonocytogenesMacrophage CellsBacterial PathogenesisBone Marrow Derived Macrophages (BMDMs)Brain Heart Infusion (BHI) MediumGentamicinBacterial InfectionRNA Extraction

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