C Kit Cd34 Markers
C Kit Cd34 Markers - Related Videos
09:00
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Cited by 15 •
2009
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
04:41
Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells
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2023
This video describes the technique of isolating c-kit positive cells from the bone marrow of a mouse. In the protocol, we will isolate c-kit positive cells from a transgenic mouse to validate c-Fos and Dusp1 alone or together as drug targets in leukemia.
11:52
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
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Cited by 8 •
2007
Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of procedures.
09:40
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
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2017
DNA-protein interactions are essential for multiple biological processes. During the evaluation of cellular functions, the analysis of DNA-protein interactions is indispensable for understanding gene regulation. Chromatin immunoprecipitation (ChIP) is a powerful tool to analyze such interactions in vivo.
FAQs
c-Kit, also called CD117, functions as a receptor for stem cell factor, linking its detection with a signaling system associated with hematopoietic cells. CD34 provides a different surface feature associated with immature blood-forming cells. Considering these biological roles helps researchers interpret staining as evidence of cellular state rather than treating either signal as an isolated label.
Their combined expression patterns provide more information than either marker alone. c-Kit reflects the presence of a stem cell factor receptor, whereas CD34 is associated with immature blood-forming cells. Comparing both signals can help distinguish developing cell populations and characterize the composition of a sample, including whether it contains cells with features associated with stem or progenitor states.
Antibodies directed against c-Kit or CD34 bind their corresponding cell-surface proteins and make those proteins detectable. In flow cytometry, the signals support analysis of labeled cells within a sample, while immunostaining reveals marker distribution in the examined material. The selected approach therefore determines whether the main outcome is cell-population characterization or visual localization.
Researchers examine bone marrow or blood samples with antibodies against the two surface proteins, using flow cytometry or immunostaining to detect expression. They then compare the resulting patterns to characterize the cells present and identify developing populations. This workflow supports assessment of sample composition and can help evaluate whether a preparation is enriched for stem or progenitor cells.
Expression patterns can indicate whether a sample contains a greater representation of cells associated with immature blood formation. Evaluating c-Kit and CD34 together helps researchers characterize that representation and assess stem-cell enrichment rather than relying on a single surface signal. This information is useful when comparing preparations or examining how cellular populations are distributed in bone marrow and blood.
The markers provide a way to characterize hematopoietic populations in studies of leukemia, tissue regeneration, and cell-based therapies. In leukemia research, expression patterns help evaluate developing or abnormal blood-forming populations. In regenerative and therapeutic work, the same measurements support assessment of selected cell preparations and help researchers track populations relevant to treatment development.