C18 Stage Tips
C18 Stage Tips - Related Videos
05:43
A STAGE Tip Procedure for Desalting and Purification of Peptides
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2026
Source:Tia Rizakos1, Jennifer Geddes-McAlister11Molecular and Cellular Biology Department, University of Guelph.This video demonstrates peptide purification through the STAGE Tip procedure. A miniature column containing resin beads bonded to long hydrocarbon chains facilitates hydrophobic interaction-based purification. Upon loading a pre-digested peptide sample into the column, the peptide fragments bind to the resin beads, allowing impurities to pass through. An elution buffer that disrupts...
08:11
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction
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Cited by 7 •
2017
Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.
10:32
Surgical Tips and Tricks for Performing Porcine Pancreas Transplantation
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Cited by 4 •
2022
The video article summarizes the technique of pancreatectomy and pancreas allotransplantation in a porcine 3-day survival model with a step-by-step description of the method and emphasis on the surgical tips and tricks to deal with the precarious and delicate porcine visceral anatomy.
01:22
Stages of Sleep
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2024
Sleep progresses through distinct stages, each characterized by specific brain wave patterns and physiological responses ranging from wakefulness to stages of non-rapid eye movement, known as non-REM, to rapid eye movement, referred to as REM. Understanding these stages helps in recognizing how sleep supports various bodily and cognitive functions. Before sleep begins, in wakefulness, the brain exhibits primarily beta waves, which are high in frequency and low in amplitude, indicating alertness...
01:22
Stages of General Anesthesia
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2024
Various sedation levels offer significant advantages in facilitating procedural interventions for patients undergoing medical or invasive surgical procedures. These levels span from anxiolysis to general anesthesia, providing a spectrum of sedative effects to cater to specific patient needs. Anxiolysis reduces anxiety and is achieved through minimal sedation, enabling patients to remain awake and responsive while feeling more at ease during the procedure. This level can benefit minor...
FAQs
Retention on C18 silica depends on nonpolar interactions with peptide regions. During loading, hydrophobic peptides remain associated with the packed material, whereas polar salts, detergents, and related contaminants pass through during washing. Increasing the organic solvent concentration weakens this retention and permits peptide recovery. This solvent-driven selectivity enables cleanup and controlled fractionation.
Conditioning the packed bed supports effective peptide retention during sample loading. Loading must bring the peptide sample into contact with the C18 silica, while washing removes polar salts, detergents, and other contaminants before recovery. When these stages are handled effectively, the recovered fraction contains fewer interfering substances and is better suited to downstream analysis.
The organic solvent concentration determines when retained peptides are released from the C18 material. Washing at a lower concentration removes passing contaminants without recovering the retained peptide fraction, whereas increasing the concentration promotes elution. Changing this condition can therefore support either collective peptide recovery or separation into fractions for subsequent analysis.
A C18 StageTip workflow follows four linked stages: condition the packed material, load the peptide sample, wash away salts, detergents, and other polar contaminants, then increase the organic solvent concentration for elution. Keeping these stages distinct helps preserve the intended retention and release behavior, improving peptide recovery while reducing contaminants in the recovered sample.
C18 StageTips are particularly useful when a protocol needs low-volume peptide cleanup without a large or elaborate format. Their miniature design and low cost make them practical for sample preparation before liquid chromatography-mass spectrometry. In this setting, cleanup and concentration can support more reliable peptide identification and protein quantification.
After effective cleanup, the recovered sample can provide more reliable peptide identification and protein quantification in downstream analyses. Reduced salts, detergents, and other contaminants help make the peptide fraction more compatible with liquid chromatography-mass spectrometry, while fractionation can separate sample components for analysis. This makes the approach relevant to biological proteomics workflows.