Calcium Retention Capacity
Calcium Retention Capacity - Related Videos
02:42
Determination of the Calcium Retention Capacity of Isolated Mitochondria
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2025
This video demonstrates the procedure to assess mitochondrial calcium retention capacity by monitoring real-time changes in fluorescence as mitochondria uptake calcium. This assay is useful for studying mitochondrial function and calcium-related processes, including apoptosis and neurodegenerative diseases.
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
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Cited by 11 •
2018
This protocol aims to describe a method to examine the Ca2+ retention capacity and Ca2+- triggered mitochondrial swelling of isolated mitochondria of SH-SY5Y cells step-by-step.
01:47
Lung Capacity
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2019
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures. There are four defined lung volume measures: tidal volume, inspiratory reserve volume, expiratory reserve volume, and residual volume. Tidal volume is the amount of air inhaled and exhaled in a normal breath. Inspiratory...
03:10
Soil Core Sample Collection for Water Retention Property Analysis
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2026
Source:Alessia J. Marchesan1, Guillermo Hernandez Ramirez11Renewable Resources, University of Alberta. This video demonstrates the technique for collecting a soil sample for hydraulic property analysis. The sampling area is cleared, and the top crust is evacuated. The sampling ring is then placed into the soil to collect the sample. The ring with the sample is then cleaned, labeled, packed, and transferred undisturbed for analysis.
11:01
Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention
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Cited by 12 •
2015
Our goals were to design, manufacture and test ferromagnetic stents for endothelial cell capture. Ten stents were tested for fracture and 10 more stents were tested for retained magnetism. Finally, 10 stents were tested in-vitro and 8 more stents were implanted in 4 pigs to show cell capture and retention.
FAQs
Calcium retention capacity links the accumulated Ca2+ load to mitochondrial membrane integrity. As mitochondria receive successive calcium inputs, the assay identifies the point at which permeability transition pore opening is associated with loss of integrity. This threshold provides a functional readout of resilience, allowing biology researchers to assess how organelles respond to calcium stress.
Repeated calcium pulses allow researchers to follow mitochondrial calcium handling over time rather than measuring the response to one exposure. Fluorescent probes track Ca2+ remaining outside the mitochondria after each addition, while the changing extramitochondrial signal reveals when sequestration can no longer continue. The cumulative amount delivered before that transition becomes the retention measurement.
Permeability transition pore opening marks the critical event used to define the assay endpoint. Before this event, mitochondria retain successive calcium inputs; afterward, membrane integrity is lost, indicating that the organelles have crossed a stress threshold. Pore opening therefore converts a calcium-handling observation into a functional measure of mitochondrial resilience and cellular health.
Changes in calcium retention capacity can reveal how mitochondrial calcium homeostasis relates to apoptosis and metabolic dysfunction. Researchers can compare the calcium load tolerated under different experimental conditions and connect altered tolerance with organelle stress. This makes the assay useful for examining whether genetic, drug-related, toxic, or disease-associated conditions disrupt mitochondrial function.
The workflow begins with isolated mitochondria or permeabilized cells, followed by repeated calcium pulses and fluorescent monitoring of extramitochondrial Ca2+. Researchers track the response until permeability transition pore opening signals loss of membrane integrity. They then record the cumulative calcium delivered up to that point as the retention-capacity outcome for the tested preparation.
These comparisons are useful when researchers need to determine whether a tested factor changes mitochondrial susceptibility to calcium stress. Measuring the cumulative load under each condition provides a common functional outcome for evaluating effects on calcium homeostasis and organelle function. The approach supports studies of genetic influences, drug effects, toxic exposures, and disease-associated mitochondrial dysfunction.