Chemical Inhibitors
Chemical Inhibitors - Related Videos
11:34
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Cited by 14 •
2014
In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.
07:44
Determining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation
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Cited by 20 •
2017
A protocol to avoid the oxidation of metallic substrates during sample transfer from an inhibited acidic solution to an X-ray photoelectron spectrometer is presented.
08:49
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
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Cited by 2 •
2019
This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.
01:52
Eukaryotic Transcription Inhibitors
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2020
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells. Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
04:00
Modelling Intratumoral Treatment with miRNA Inhibitors: A Method to Analyze the Effect of miRNA Inhibitors on Tumor Growth in Murine Model
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2023
This video demonstrates the in vivo delivery of miRNA inhibitors as therapeutic agents against tumor growth in orthotopic mouse models. The intratumoral administration of synthetic miRNA inhibitors or antagomiRs allows them to bind and inhibit endogenous miRNAs, thereby causing mRNA upregulation and suppression of tumor growth.
FAQs
Chemical inhibitor effects often depend on concentration, so researchers examine responses across a range rather than relying on a single dose. This produces a dose-response relationship showing how strongly the target or biological system changes as exposure increases. Comparing these patterns helps characterize potency and supports interpretation of cellular or organismal effects.
The distinction depends on how the inhibitor interacts with its target. Reversible inhibition can be relieved when the inhibitor is no longer present, whereas irreversible inhibition involves a lasting chemical modification. This difference matters experimentally because it affects how long activity remains suppressed and how researchers interpret recovery, persistence, and target engagement.
They can compete with a natural substrate at a target site, alter the molecule’s shape, or chemically modify it. These mechanisms reduce activity through different molecular routes, even when the observable outcome is similar. Identifying the route helps researchers connect a change in protein function with a specific molecular interaction.
Specificity indicates how selectively an inhibitor affects its intended molecular target compared with other biological components. Researchers examine it because a cellular response may reflect the targeted pathway or additional affected molecules. Evaluating specificity therefore strengthens conclusions about gene and protein function and helps distinguish a direct mechanism from broader system-level effects.
Researchers can vary inhibitor concentration and measure the resulting change in molecular, cellular, or organismal activity. They then compare dose-response patterns and consider whether suppression is reversible or persistent. Assessing specificity adds another layer of interpretation. Together, these observations connect inhibitor exposure with target behavior and help determine whether the effect supports a proposed pathway.
They are useful when researchers need to reduce a molecular activity and observe what changes downstream. Suppressing a protein or signaling component can help reveal its contribution to a cellular process or pathway. This approach supports investigations of gene and protein function, especially when responses can be measured across concentrations or in whole-cell systems.
Inhibitors can reveal molecular targets whose activity influences a biological process, making them valuable tools in therapeutic research. Dose-response measurements help relate exposure to biological effect, while specificity and system-level testing provide evidence about how selectively the target can be controlled. Studies in whole-cell or organismal systems extend findings beyond isolated molecular activity.